高效液相色谱法同时测定3种不同剂型六味地黄丸中4种成分的含量

王灵霞; 朱东亮; 柴逸峰

doi:10.3969/j.issn.1006-0111.2013.04.016

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高效液相色谱法同时测定3种不同剂型六味地黄丸中4种成分的含量

王灵霞, 朱东亮, 柴逸峰

文章导航 > 药学实践与服务 > 2013 > 31(4): 296-299

王灵霞, 朱东亮, 柴逸峰. 高效液相色谱法同时测定3种不同剂型六味地黄丸中4种成分的含量[J]. 药学实践与服务, 2013, 31(4): 296-299. doi: 10.3969/j.issn.1006-0111.2013.04.016

引用本文:

WANG Ling-xia, ZHU Dong-liang, CHAI Yi-feng. Simultaneous determination of contents of four components in three different dosage forms of Liuweidihuang by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 296-299. doi: 10.3969/j.issn.1006-0111.2013.04.016

Citation:

WANG Ling-xia, ZHU Dong-liang, CHAI Yi-feng. Simultaneous determination of contents of four components in three different dosage forms of Liuweidihuang by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 296-299. doi: 10.3969/j.issn.1006-0111.2013.04.016

高效液相色谱法同时测定3种不同剂型六味地黄丸中4种成分的含量

doi: 10.3969/j.issn.1006-0111.2013.04.016

1.
新疆军区乌鲁木齐综合库药材分库, 新疆乌鲁木齐 830002
2.
新疆军区联勤部药品仪器检验所, 新疆乌鲁木齐 830063
3.
第二军医大学药学院, 上海 200433

Simultaneous determination of contents of four components in three different dosage forms of Liuweidihuang by HPLC

1.
Department of Pharmaceutical equipment, Urumqi Integrated Warehouse of Xinjiang Military Region, Urumqi 830002, China
2.
Institute for Drug and Instrument Control of Xinjiang Military Region, Urumqi 830063, China
3.
School of Pharmacy, Second military Medical University, Shanghai 200433, China

摘要: 目的建立HPLC法测定3种不同剂型六味地黄丸中没食子酸、马钱苷、芍药苷和丹皮酚含量的方法。方法色谱柱为Agilent ZorbaxSB-C₁₈柱(250 mm×4.6 mm,5 μm);流动相为二元梯度系统,其中溶剂A为乙腈,溶剂B为0.1%甲酸水溶液,梯度洗脱:(0~3 min,A为3%;3 min,A为5%;28 min,A为22%;40 min,A为60%),流速:1 ml/min; 柱温:25℃;检测波长:240 nm;自动进样10 μl。结果没食子酸、马钱苷、芍药苷和丹皮酚浓度分别在6.296~318.9 μg/ml(r=0.999 8)、1.952~99.04 μg/ml(r=0.999 7)、6.186~309.3 μg/ml(r=0.999 8)和7.147~214.4 μg/ml(r=0.999 7)范围内呈现良好的线性,精密度实验RSD均小于2%,加样回收率98.2%~102.3%。结论该测定方法简便、准确,分离效果好,能同时测定4种成分的含量,适用于不同剂型六味地黄丸的质量控制。
- 六味地黄丸 /
- 高效液相色谱法 /
- 含量测定 /
- 没食子酸 /
- 马钱苷 /
- 芍药苷 /
- 丹皮酚
Abstract: Objective To develop a new high performance liquid chromatography (HPLC) method for simultaneous determination of four major components (gallic acid, loganin, paeoniflorin and paeonol) in different dosage forms of Liuweidihuang. Methods The chromatography condition was with Agilent Zorbax SB-C₁₈ column (4.6×250 mm, 5 μm);mobile phase was A:ACN, B:0.1% formic acid, gradient elution, 0~3 min, A:3%~5%;3~18 min, A:5%~22%;18~60 min, A:22%~60%, flow speed was 1.0 ml/min, temperature of column was 25℃, inject volume was 10 μl, detection wavelength was 240 nm. Results The linearity was obtained over 6.296~318.9 μg/ml(r=0.999 8)for gallic acid, 1.952~99.04 μg/ml(r=0.999 7)for loganin, 6.186~309.3 μg/ml(r=0.999 8)for paeoniflorin and 7.147~214.4 μg/ml(r=0.999 7)for paeonol.The RSDs of precision of the samples were both less than 2%.The average recovery was between 98.2%~102.3%. Conclusion The present method, with satisfactory efficacy, was accurate and simple which could simultaneously determine four components, and could be used for quality control of different dosage forum of Liuweidihuang.
- Liuweidihuang /
- HPLC /
- content determination /
- gallic acid /
- loganin /
- paeoniflorin /
- paeonol

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高效液相色谱法同时测定3种不同剂型六味地黄丸中4种成分的含量

doi: 10.3969/j.issn.1006-0111.2013.04.016

1. 新疆军区乌鲁木齐综合库药材分库, 新疆乌鲁木齐 830002

2. 新疆军区联勤部药品仪器检验所, 新疆乌鲁木齐 830063

3. 第二军医大学药学院, 上海 200433

收稿日期: 2013-05-27
修回日期: 2013-06-13

关键词:

马钱苷 /

芍药苷 /

丹皮酚

摘要: 目的建立HPLC法测定3种不同剂型六味地黄丸中没食子酸、马钱苷、芍药苷和丹皮酚含量的方法。方法色谱柱为Agilent ZorbaxSB-C₁₈柱(250 mm×4.6 mm,5 μm);流动相为二元梯度系统,其中溶剂A为乙腈,溶剂B为0.1%甲酸水溶液,梯度洗脱:(0~3 min,A为3%;3 min,A为5%;28 min,A为22%;40 min,A为60%),流速:1 ml/min; 柱温:25℃;检测波长:240 nm;自动进样10 μl。结果没食子酸、马钱苷、芍药苷和丹皮酚浓度分别在6.296~318.9 μg/ml(r=0.999 8)、1.952~99.04 μg/ml(r=0.999 7)、6.186~309.3 μg/ml(r=0.999 8)和7.147~214.4 μg/ml(r=0.999 7)范围内呈现良好的线性,精密度实验RSD均小于2%,加样回收率98.2%~102.3%。结论该测定方法简便、准确,分离效果好,能同时测定4种成分的含量,适用于不同剂型六味地黄丸的质量控制。

English Abstract

Simultaneous determination of contents of four components in three different dosage forms of Liuweidihuang by HPLC

1. Department of Pharmaceutical equipment, Urumqi Integrated Warehouse of Xinjiang Military Region, Urumqi 830002, China

2. Institute for Drug and Instrument Control of Xinjiang Military Region, Urumqi 830063, China

3. School of Pharmacy, Second military Medical University, Shanghai 200433, China

Received Date: 2013-05-27
Rev Recd Date: 2013-06-13

Keywords:

Abstract: Objective To develop a new high performance liquid chromatography (HPLC) method for simultaneous determination of four major components (gallic acid, loganin, paeoniflorin and paeonol) in different dosage forms of Liuweidihuang. Methods The chromatography condition was with Agilent Zorbax SB-C₁₈ column (4.6×250 mm, 5 μm);mobile phase was A:ACN, B:0.1% formic acid, gradient elution, 0~3 min, A:3%~5%;3~18 min, A:5%~22%;18~60 min, A:22%~60%, flow speed was 1.0 ml/min, temperature of column was 25℃, inject volume was 10 μl, detection wavelength was 240 nm. Results The linearity was obtained over 6.296~318.9 μg/ml(r=0.999 8)for gallic acid, 1.952~99.04 μg/ml(r=0.999 7)for loganin, 6.186~309.3 μg/ml(r=0.999 8)for paeoniflorin and 7.147~214.4 μg/ml(r=0.999 7)for paeonol.The RSDs of precision of the samples were both less than 2%.The average recovery was between 98.2%~102.3%. Conclusion The present method, with satisfactory efficacy, was accurate and simple which could simultaneously determine four components, and could be used for quality control of different dosage forum of Liuweidihuang.

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高效液相色谱法同时测定3种不同剂型六味地黄丸中4种成分的含量

doi: 10.3969/j.issn.1006-0111.2013.04.016

Simultaneous determination of contents of four components in three different dosage forms of Liuweidihuang by HPLC

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高效液相色谱法同时测定3种不同剂型六味地黄丸中4种成分的含量

doi: 10.3969/j.issn.1006-0111.2013.04.016

1. 新疆军区乌鲁木齐综合库药材分库, 新疆 乌鲁木齐 830002 2. 新疆军区联勤部药品仪器检验所, 新疆 乌鲁木齐 830063 3. 第二军医大学药学院, 上海 200433

English Abstract

Simultaneous determination of contents of four components in three different dosage forms of Liuweidihuang by HPLC

1. Department of Pharmaceutical equipment, Urumqi Integrated Warehouse of Xinjiang Military Region, Urumqi 830002, China 2. Institute for Drug and Instrument Control of Xinjiang Military Region, Urumqi 830063, China 3. School of Pharmacy, Second military Medical University, Shanghai 200433, China

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目录

1. 新疆军区乌鲁木齐综合库药材分库, 新疆乌鲁木齐 830002

2. 新疆军区联勤部药品仪器检验所, 新疆乌鲁木齐 830063

3. 第二军医大学药学院, 上海 200433

1. Department of Pharmaceutical equipment, Urumqi Integrated Warehouse of Xinjiang Military Region, Urumqi 830002, China

2. Institute for Drug and Instrument Control of Xinjiang Military Region, Urumqi 830063, China

3. School of Pharmacy, Second military Medical University, Shanghai 200433, China