α-Glucosidase inhibitory activity of extracts from roots of Salacia amplifolia Merr.ex ChunF.C.
-
摘要: 目的 研究阔叶五层龙根各提取物体外α-糖苷酶抑制活性。 方法 将阔叶五层龙根80%乙醇提取物依次用石油醚、乙酸乙酯、正丁醇萃取,得到4个不同极性部位,并对80%乙醇总提取物与4个不同极性部位进行α-葡萄糖苷酶和α-淀粉酶体外抑制活性筛选。 结果 阔叶五层龙根各提取物均具有一定的α-葡萄糖苷酶和α-淀粉酶体外抑制活性,其中对α-葡萄糖苷酶的抑制活性大小:正丁醇部位 > 石油醚部位 > 乙酸乙酯 > 总提取物;对α-淀粉酶的抑制活性大小:石油醚部位 > 正丁醇部位 > 总提取物 > 乙酸乙酯部位。 结论 阔叶五层龙根各提取物均具有较好的α-糖苷酶抑制活性和很好的开发价值。Abstract: Objective To investigate different extracts of Salacia amplifolia Merr.ex ChunF.C on inhibitory activity to α-glucosidase. Methods The 80% aqueous ethanol extracts from roots of S.amplifolia, which were partitioned sequentially with petroleum ether, EtOAc and n-BuOH respectively to yield four different fractions. And the inhibitory activity of these five extracts for the α-glucosidase and α-amylase were determined by in vitro experiments. Results Five kinds of extracts showed moderate inhibitory activity at the same time and close to acarbose to a certain degree.Inhibitory activity of α-glucosidase were, n-BuOH fraction > petroleum ether fraction > EtOAc fraction > total fraction.The inhibitory of α-amylase activity followed by petroleum ether fraction > n-BuOH fraction > total fraction > EtOAc fraction, respectively. Conclusion The extracts from S.amplifolia had good inhibitory activity on α-glucosidase, which could be exploited the diabetes drugs as α-glucosidases inhibitors in future.
-
Key words:
- Salacia amplifolia /
- α-glucosidase /
- inhibitory activity
-
[1] Lraj H,Vida R.Chronic complications of diabetes mellitus in newly diagnosed patients[J].Int J Diabetes Mell,2010,2(1):61. [2] Mohamed A, Barra R, Naziaa U, et al. Cognitive impairment in type 2 diabetes mellitus[J].Int J Diabetes Mell,2011,3(2):181. [3] Kim KY,Nama KA,Kurihara H,et al.Potent α-glucosidase inhibitors purified from the red alga Grateloupia elliptica[J]. Phytochemistry,2008,69(16):2820. [4] Ortiz-Andrade RR,García-Jiménez S,Castillo -España P,et al. α-Glucosidase inhibitory activity of the methanolic extract from Tournefortia hartwegiana:An anti-hyperglycemic agent[J]. J Ethnopharmacol,2007,109(1):48. [5] Mbaze LM,Poumale HM,Wansi JD,et al. α-Glucosidase inhibitory pentacyclic triterpenes from the stem bark of Fagara tessmannii (Rutaceae)[J]. Phytochemistry,2007,68(5):591. [6] Huang YN,Zhao YL,Gao XL,et al.Intestinal α-glucosidase inhibitory activity and toxicological evaluation of Nymphaea stellata flowers extract[J]. J Ethnopharmacol,2010,131(2):306. [7] 中国科学院中国植物志编辑委员会.中国植物志[M]. 第46卷,北京:科学出版社, 1981,46:6. [8] 广东省植物研究所.海南植物志[M]. 北京:科学出版社,1977,443. [9] 郭澄,张纯.国外植物药治疗糖尿病的研究进展[J]. 国外医学:中医中药分册,1997,19(3):51. [10] 袁干军,田育望,王志琪. 阔叶五层龙根乙醇提取物的降血糖作用[J]. 中药新药与临床药理,2005,16(4):253. -
点击查看大图
计量
- 文章访问数: 2717
- HTML全文浏览量: 185
- PDF下载量: 295
- 被引次数: 0
下载:
