Proliferation inhibition of total alkaloids from Pinellia Ternata in human lung cancer cells
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摘要: 目的 探讨半夏总生物碱(total alkaloids from Pinellia Ternata,TATP)对人肺癌细胞株A549增殖的影响。 方法 采用四甲基偶氮唑盐(methyl thiazolil tetracolium,MTT)比色法以及集落形成率实验,检测不同浓度的TATP对A549细胞株的生长抑制作用。应用单细胞凝胶电泳分析检测TATP导致A549细胞的DNA损伤情况。 结果 人肺癌细胞A549经TATP处理后,其体外增殖能力明显下降且与药物的剂量、加药时间呈正相关,与对照组比较,差异均有统计学意义。单细胞凝胶电泳(Single cell gel electrophoresis, SCGE)中,TATP组细胞尾DNA含量、尾长及尾动量与对照组相比差异显著(P<0.01),且呈现浓度依赖性。 结论 在体外培养的条件下,TATP能明显抑制A549细胞增殖,其机制可能与DNA的损伤作用有关。Abstract: Objective To investigate the proliferation inhibition of total alkaloids from Pinellia ternate (TATP) in human lung cancer line A549. Methods The proliferation inhibition of human lung cancer cell line A549 treated with TATP was measured with methyl thiazolil tetracolium colorimetic method and plate clone formation assay. The DNA damage of A549 cell induced by TATP was detected with the single cell gel electrophoresis (SCGE). Results The proliferation in A549 cell treated with TATP was decreased significantly compared with control group and the proliferation inhibition was positively correlated to the TATP concentration and the reaction time. The difference between the control group and the TATP experimental groups was significant in statistic. Compared with the control group, the tail DNA content, the tail length and the tail movement of the TATP experimental groups were different significantly and presented a dose-effect relationship. Conclusion TATP could inhibit the proliferation of A549 cells in vitro, which might be correlated to the DNA damage induced by TAPT.
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