LC-MS/MS测定大鼠血浆中虎杖苷浓度

丁雪鹰; 侯晓丽; 孙铭学; 肖凯

doi:10.3969/j.issn.1006-0111.2012.01.012

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

LC-MS/MS测定大鼠血浆中虎杖苷浓度

丁雪鹰, 侯晓丽, 孙铭学, 肖凯

文章导航 > 药学实践与服务 > 2012 > 30(1): 45-48

丁雪鹰, 侯晓丽, 孙铭学, 肖凯. LC-MS/MS测定大鼠血浆中虎杖苷浓度[J]. 药学实践与服务, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012

引用本文:

丁雪鹰, 侯晓丽, 孙铭学, 肖凯. LC-MS/MS测定大鼠血浆中虎杖苷浓度[J]. 药学实践与服务, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012

DING Xue-ying, HOU Xiao-li, SUN Ming-xue, XIAO Kai. Determination of polydatin in rats plasma by LC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012

Citation:

DING Xue-ying, HOU Xiao-li, SUN Ming-xue, XIAO Kai. Determination of polydatin in rats plasma by LC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012

LC-MS/MS测定大鼠血浆中虎杖苷浓度

doi: 10.3969/j.issn.1006-0111.2012.01.012

1.
第二军医大学药学院药剂学教研室,上海 200433
2.
第二军医大学海医系毒物药物研究室,上海 200433

基金项目: 上海市科委中药现代化项目(08DZ1971504);国家自然科学基金(20872179,30472141).

Determination of polydatin in rats plasma by LC-MS/MS

1.
Department of Pharmaceutics, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
2.
Laboratory of Toxicology & Pharmacology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China

摘要: 目的建立测定大鼠血浆中虎杖苷浓度的LC-MS/MS方法。方法采用LC-MS/MS测定大鼠血浆中虎杖苷的浓度,以二苯乙烯苷为内标,血浆样品用乙腈沉淀蛋白,色谱柱为Agilent Zorbax SB-C₁₈ (100 mm×2.1 mm, 3.5 μm),流动相为甲醇-乙腈-0.1%甲酸水溶液(18∶15∶67),流速0.3 ml/min,柱温30 ℃。结果虎杖苷的线性范围为1.0~5 000.0 ng/ml (r=0.998 4),最低定量检测浓度为1.0
- 虎杖苷 /
- LC-MS/MS /
- 血药浓度
Abstract: Objective To establish a LC-MS/MS method for determination of polydatin concentration in rats plasma. Methods The plasma concentration of polydatin was determined by LC-MS/MS. Using stilbene glucoside as internal standard, after precipitation of the plasma proteins with methanol, the analytes were separated on an agilent zorbax SB-C₁₈ reversed-phase column with methanol-acetonitrile- 0.1% formic acid (18 : 15 : 67, v/v) and detected by electrospray ionization (ESI) mass spectrometry in negative multiple reaction monitoring (MRM) mode. The flow rate was 0.3 ml/min. Column temperature was maintained at 30 ℃. Results The calibration curves with good linearity (r=0.998 4 for plasma sample) were obtained in the range of 1.0~5000.0 ng/ml for polydatin. The lower limit of quantification (LLOQ) was 1.0 ng/ml. Recoveries were around 78% for the extraction from rats plasma, and good precision and accuracy were achieved. The intra-day and inter-day RSD were both less than 15.0%. This method was feasible for the evaluation of pharmacokinetic profiles of polydatin in rats. Conclusion This method was simple, selective and sensitive. It's suitable for the pharmacokinetic research of polydatin in rats plasma.
- polydatin /
- LC-MS/MS /
- plasma drug concentration

[1]	肖凯,宣利江,徐亚明,等.虎杖的化学成分研究[J].中国药学杂志, 2003,38(1):12.
[2]	高守红,杨少麟,范国荣. 虎杖苷的研究进展[J].药学实践杂志,2005,23(3):145.
[3]	李蓟龙,尹京湘,张力,等. 虎杖中白藜芦醇对豚鼠左心室流出道自律组织电活动的影响[J]. 中国应用生理学杂志,2010,26(3):311,364.
[4]	刘铭,刘华,张国平,等. 虎杖苷对脑出血性损伤大鼠血清白细胞介素-1β和脑组织Bcl-2蛋白表达的影响[J]. 中草药,2010, 41(12):2010.
[5]	Gao S, Fan G, Hong Z, et al. HPLC determination of polydatin in rat biological matrices: application to pharmacokinetic studies[J]. J Pharmaceut Biomed,2006, 41(1): 240.
[6]	吕春艳,张兰桐,袁志芳,等. 虎杖苷在大鼠体内的药动学特点和组织分布研究[J].中草药,2006,38(2):235.
[7]	林建海,李笑宏,唐跃年,等. 白藜芦醇苷的HPLC法分析及其在苗猪体内的药代动力学研究[J]. 药物分析杂志, 2001,21(05):325.
[8]	杨润涛,周四元,章翔,等.液相色谱-串联质谱法同时测定血浆中白藜芦醇苷及其代谢产物[J]. 分析化学,2007,35(9):1309.
[9]	Zhou S, Yang R,Teng Z,et al. Dose-dependent absorption and metabolism of trans-polydatin in rats[J]. J Agr Food Chem, 2009,57(11): 4572.

[1]	桂明珠, 李静, 谢晓恬, 李志玲. 伏立康唑治疗儿童侵袭性真菌感染血药浓度与疗效相关性研究 . 药学实践与服务, 2022, 40(4): 359-363. doi: 10.12206/j.issn.1006-0111.202106075
[2]	侯文洁, 张亮, 李翔宇, 王洁. CYP2C19基因多态性对PCI术后患者氯吡格雷血药浓度、血小板抑制率和安全性的影响 . 药学实践与服务, 2021, 39(5): 472-475. doi: 10.12206/j.issn.1006-0111.202008029
[3]	方慧君, 段烨, 张春丽, 黄隆基, 王荣. LC-MS/MS法测定红景天胶囊中红景天苷含量的方法 . 药学实践与服务, 2021, 39(2): 157-159. doi: 10.12206/j.issn.1006-0111.202007059
[4]	常瑞蕊, 陈卫东, 周婷婷. 用LC-MS/MS法同时测定栀子中3种环烯醚萜苷类成分的含量 . 药学实践与服务, 2019, 37(1): 19-22. doi: 10.3969/j.issn.1006-0111.2019.01.005
[5]	陈浩, 李新方, 孙渺, 张钢, 杨峰. 用LC-MS/MS法测定大鼠血浆中大蒜皂苷PIEB的浓度及其体内药动学研究 . 药学实践与服务, 2019, 37(1): 37-41. doi: 10.3969/j.issn.1006-0111.2019.01.009
[6]	陈碧, 黄晓亮, 李辉. 反相高效液相色谱-柱前衍生法测定人血清中丙戊酸浓度 . 药学实践与服务, 2016, 34(3): 255-257. doi: 10.3969/j.issn.1006-0111.2016.03.016
[7]	吴丽红, 储忠英, 刘惠军. HPLC-DAD法同时测定肝八味胶囊中4种成分的含量 . 药学实践与服务, 2015, 33(3): 250-252. doi: 10.3969/j.issn.1006-0111.2015.03.015
[8]	恽芸蕾, 高守红, 樊成辉, 缪海均. 米格列奈钙片健康人体药动学研究 . 药学实践与服务, 2014, 32(1): 45-48. doi: 10.3969/j.issn.1006-0111.2014.01.011
[9]	杨小英, 马启珍. LC-MS-MS法测定犬体内血浆中埃索美拉唑药物浓度 . 药学实践与服务, 2013, 31(1): 61-63. doi: 10.3969/j.issn.1006-0111.2013.01.018
[10]	段迪, 凌迎春, 周月琴, 王玲英. 同等剂量喹硫平治疗精神分裂症的血药浓度与临床疗效的关系 . 药学实践与服务, 2012, 30(3): 218-219. doi: 10.3969/j.issn.1006-0111.2012.03.017
[11]	王晶, 金磊, 熊礼燕, 王庭芳, 郑莉萍, 张宁, 邹豪, 张川. HPLC-MS/MS测定大鼠血浆中丹参素钠的浓度 . 药学实践与服务, 2011, 29(2): 109-110,130.
[12]	姜楠, 杨永革, 许雪廷, 赵钢涛. LC-MS-MS法测定人血浆中阿托伐他汀浓度 . 药学实践与服务, 2011, 29(1): 15-16,70.
[13]	陈咏, 林晨, 张晶, 邱彬, 周欣, 宋洪涛. UPLC-MS/MS测定beagle犬内西罗莫司血药浓度 . 药学实践与服务, 2011, 29(5): 353-355,375.
[14]	王慧, 张国庆. 反相高效液相色谱法测定罗哌卡因血药浓度 . 药学实践与服务, 2007, (4): 238-240.
[15]	蔡少华, 龙冬兰, 蔡韬艺. 回顾性分析357例次小儿抗癫痫药物血药浓度 . 药学实践与服务, 2005, (4): @-@.
[16]	高守红, 杨少麟, 范国荣. 虎杖苷的研究进展 . 药学实践与服务, 2005, (3): 145-147.
[17]	余爱荣. 高效液相色谱法测定噻氯匹定的血药浓度 . 药学实践与服务, 1998, (4): 228-230.
[18]	陈坚, 方维军, 张灯玲, 丁宗政, 陈睿. 反相高效液相色谱法测定对乙酰氨基酚血药浓度 . 药学实践与服务, 1998, (5): 296-298.
[19]	杨丽英, 李景玉, 卢清林, 李红宇. 高效液相色谱法监测血清中卡马西平血药浓度50例分析 . 药学实践与服务, 1997, (2): 99-100.
[20]	柳正良, 李名秀, 崔志平. 高效离子对液相色谱法测定促甲状腺激素释放激素(TRH)血药浓度的研究 . 药学实践与服务, 1995, (4): 234-236.

点击查看大图

计量

文章访问数: 2499
HTML全文浏览量: 179
PDF下载量: 394
被引次数: 0

LC-MS/MS测定大鼠血浆中虎杖苷浓度

doi: 10.3969/j.issn.1006-0111.2012.01.012

1. 第二军医大学药学院药剂学教研室,上海 200433

2. 第二军医大学海医系毒物药物研究室,上海 200433

基金项目: 上海市科委中药现代化项目(08DZ1971504);国家自然科学基金(20872179,30472141).

收稿日期: 2011-02-10
修回日期: 2011-12-21

关键词:

虎杖苷 /

LC-MS/MS /

血药浓度

摘要: 目的建立测定大鼠血浆中虎杖苷浓度的LC-MS/MS方法。方法采用LC-MS/MS测定大鼠血浆中虎杖苷的浓度,以二苯乙烯苷为内标,血浆样品用乙腈沉淀蛋白,色谱柱为Agilent Zorbax SB-C₁₈ (100 mm×2.1 mm, 3.5 μm),流动相为甲醇-乙腈-0.1%甲酸水溶液(18∶15∶67),流速0.3 ml/min,柱温30 ℃。结果虎杖苷的线性范围为1.0~5 000.0 ng/ml (r=0.998 4),最低定量检测浓度为1.0

English Abstract

Determination of polydatin in rats plasma by LC-MS/MS

1. Department of Pharmaceutics, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

2. Laboratory of Toxicology & Pharmacology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China

Received Date: 2011-02-10
Rev Recd Date: 2011-12-21

Keywords:

Abstract: Objective To establish a LC-MS/MS method for determination of polydatin concentration in rats plasma. Methods The plasma concentration of polydatin was determined by LC-MS/MS. Using stilbene glucoside as internal standard, after precipitation of the plasma proteins with methanol, the analytes were separated on an agilent zorbax SB-C₁₈ reversed-phase column with methanol-acetonitrile- 0.1% formic acid (18 : 15 : 67, v/v) and detected by electrospray ionization (ESI) mass spectrometry in negative multiple reaction monitoring (MRM) mode. The flow rate was 0.3 ml/min. Column temperature was maintained at 30 ℃. Results The calibration curves with good linearity (r=0.998 4 for plasma sample) were obtained in the range of 1.0~5000.0 ng/ml for polydatin. The lower limit of quantification (LLOQ) was 1.0 ng/ml. Recoveries were around 78% for the extraction from rats plasma, and good precision and accuracy were achieved. The intra-day and inter-day RSD were both less than 15.0%. This method was feasible for the evaluation of pharmacokinetic profiles of polydatin in rats. Conclusion This method was simple, selective and sensitive. It's suitable for the pharmacokinetic research of polydatin in rats plasma.

丁雪鹰, 侯晓丽, 孙铭学, 肖凯. LC-MS/MS测定大鼠血浆中虎杖苷浓度[J]. 药学实践与服务, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012

引用本文:

丁雪鹰, 侯晓丽, 孙铭学, 肖凯. LC-MS/MS测定大鼠血浆中虎杖苷浓度[J]. 药学实践与服务, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012

Citation: