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用HPLC法测定丹参素棕榈醇酯脂质体药物含量

王晶,  张川,  栗意,  任金妹,  唐扣明

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文章导航 > 药学实践与服务  > 2019 >  37(5): 450-452,480
王晶, 张川, 栗意, 任金妹, 唐扣明. 用HPLC法测定丹参素棕榈醇酯脂质体药物含量[J]. 药学实践与服务, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
引用本文: 王晶, 张川, 栗意, 任金妹, 唐扣明. 用HPLC法测定丹参素棕榈醇酯脂质体药物含量[J]. 药学实践与服务, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
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WANG Jing, ZHANG Chuan, LI Yi, REN Jinmei, TANG Kouming. Determination of palmitul 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate liposome by HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
Citation: WANG Jing, ZHANG Chuan, LI Yi, REN Jinmei, TANG Kouming. Determination of palmitul 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate liposome by HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
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用HPLC法测定丹参素棕榈醇酯脂质体药物含量

doi: 10.3969/j.issn.1006-0111.2019.05.012
  • 1.

    复旦大学附属中山医院青浦分院药剂科, 上海 201700

  • 2.

    上海大学医学院, 上海 200003

  • 3.

    上海健康医学院附属周浦医院, 上海 201318

基金项目: 上海市卫生和计划生育委员会科研项目资助（20164Y0101）

Determination of palmitul 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate liposome by HPLC method

  • 1.

    Department of Pharmacy, Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai 201700, China

  • 2.

    Department of Medicine, Shanghai University, Shanghai 200003, China

  • 3.

    Zhoupu Hospital Affiliated to Shanghai University of Medicine & Health Sciences, Shanghai 201318, China

  • 摘要: 目的 建立HPLC法测定丹参素棕榈醇酯脂质体中药物含量的方法。 方法 采用Agilent ZORBAX Eclipse Plus C18柱（4.6 mm×200 mm，5 μm）；以乙腈-水（50∶50）含0.02%甲酸和0.02%三乙胺水、以pH 6~7的溶液为流动相，流速为1 ml/min，检测波长为280 nm，柱温为室温。 结果 本色谱条件下丹参素棕榈醇酯与辅料及溶剂分离度符合要求，在0.05~0.45 mg/ml范围内线性关系良好（r=0.999 5，n=5），回收率在96%~102%之间，日内及日间RSD均小于2%（n=5）。3批样品的含量分别为97.81%、101.20%、98.53%。 结论 该方法准确可靠、简单快速，可用于丹参素棕榈醇酯脂质体含量的测定。
    Abstract: Objective To establish a HPLC method for the assay of palmitul 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate (PDSS) in liposomes. Methods The separation was performed with an Agilent ZORBAX Eclipse Plus C18 column (4.6 mm×200 mm,5 μm) at room temperature.The mobile phase was acetonitrile-aqueous solution (500 ml water + 0.1 ml triethylamine + 0.1 ml formic acid,pH=6-7) (50:50) with the flow rate of 1 ml/min.Zidovudine palmitate was detected at 280 nm. Results PDSS had a good linear recovery between 0.05-0.45 mg/ml.The intra-day and inter-day RSD were <2%.The extracted recovery was in the range of 96% to 102%.The assay results for three batches were 97.81%,101.20% and 98.53%. Conclusion This method is simple,accurate and reliable.It can be used for the determination of PDSS in liposomes.
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  • [1] 史生辉, 石飞, 雷琼, 王亚峰, 吴雪花.  青藏高原肺结核合并念珠菌感染患者的病原菌分布特点及耐药率分析 . 药学实践与服务, 2024, 42(6): 260-262, 272. doi: 10.12206/j.issn.2097-2024.202304014
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出版历程
  • 收稿日期:  2019-02-14
  • 修回日期:  2019-05-06

用HPLC法测定丹参素棕榈醇酯脂质体药物含量

doi: 10.3969/j.issn.1006-0111.2019.05.012
  • 1. 复旦大学附属中山医院青浦分院药剂科, 上海 201700
  • 2. 上海大学医学院, 上海 200003
  • 3. 上海健康医学院附属周浦医院, 上海 201318
    • 基金项目:  上海市卫生和计划生育委员会科研项目资助（20164Y0101）
  • 收稿日期: 2019-02-14
  • 修回日期: 2019-05-06

摘要: 目的 建立HPLC法测定丹参素棕榈醇酯脂质体中药物含量的方法。 方法 采用Agilent ZORBAX Eclipse Plus C18柱（4.6 mm×200 mm，5 μm）；以乙腈-水（50∶50）含0.02%甲酸和0.02%三乙胺水、以pH 6~7的溶液为流动相，流速为1 ml/min，检测波长为280 nm，柱温为室温。 结果 本色谱条件下丹参素棕榈醇酯与辅料及溶剂分离度符合要求，在0.05~0.45 mg/ml范围内线性关系良好（r=0.999 5，n=5），回收率在96%~102%之间，日内及日间RSD均小于2%（n=5）。3批样品的含量分别为97.81%、101.20%、98.53%。 结论 该方法准确可靠、简单快速，可用于丹参素棕榈醇酯脂质体含量的测定。

English Abstract

王晶, 张川, 栗意, 任金妹, 唐扣明. 用HPLC法测定丹参素棕榈醇酯脂质体药物含量[J]. 药学实践与服务, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
引用本文: 王晶, 张川, 栗意, 任金妹, 唐扣明. 用HPLC法测定丹参素棕榈醇酯脂质体药物含量[J]. 药学实践与服务, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
shu
WANG Jing, ZHANG Chuan, LI Yi, REN Jinmei, TANG Kouming. Determination of palmitul 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate liposome by HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
Citation: WANG Jing, ZHANG Chuan, LI Yi, REN Jinmei, TANG Kouming. Determination of palmitul 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate liposome by HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
shu

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