Research on the determination of total flavonoids in compound Yinchen mixture
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摘要: 目的 建立紫外-可见分光光度法测定复方茵陈合剂中总黄酮含量。 方法 以芦丁作为对照，依次用直接测定法、氯化铝-醋酸钠显色法、硝酸铝显色法，探究最优测定方法。 结果 本品最优显色法为硝酸铝显色法，测定波长为508 nm，在0.012 5~0.062 6 g/L范围内线性关系良好，线性回归方程为Y=12.127 30 X+0.000 14（r=0.999 9），加样回收率平均值为99.49%（n=9，RSD为0.84%）。 结论 硝酸铝显色法可作为复方茵陈合剂中总黄酮的含量测定方法，该法简便、快速、稳定可靠。Abstract: Objective To establish a method for the determination of the total flavonoids content in compound Yinchen mixture by UV spectrophotometry. Methods Using rutin as comparison, three coloration methods were studied to find the optimal assay method. Results The sample was detected at 508 nm wavelength by NaNO2-Al(NO3)3-NaOH reaction with rutin as reference. The rutin content had a liner relationship in the range of 0.012 5-0.062 6 g/L (n=9,r=0.999 9), and the average recovery rate was 99.49% with RSD of 0.84%. Conclusion The NaNO2-Al(NO3)3-NaOH coloration method is proved to be simple, quick, stable and reliable for the determination of total flavonoids in compound Yinchen mixture.
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