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氟尿嘧啶对结直肠癌RKO细胞SHP2基因表达的影响

王洁,  黄岩,  张川,  马兴元,  常文军,  陆一鸣

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文章导航 > 药学实践与服务  > 2017 >  35(6): 512-515
王洁, 黄岩, 张川, 马兴元, 常文军, 陆一鸣. 氟尿嘧啶对结直肠癌RKO细胞SHP2基因表达的影响[J]. 药学实践与服务, 2017, 35(6): 512-515. doi: 10.3969/j.issn.1006-0111.2017.06.008
引用本文: 王洁, 黄岩, 张川, 马兴元, 常文军, 陆一鸣. 氟尿嘧啶对结直肠癌RKO细胞SHP2基因表达的影响[J]. 药学实践与服务, 2017, 35(6): 512-515. doi: 10.3969/j.issn.1006-0111.2017.06.008
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WANG Jie, HUANG Yan, ZHANG Chuan, MA Xingyuan, CHANG Wenjun, LU Yiming. Effect of 5-FU on the expression of SHP2 gene in colorectal cancer cells RKO[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(6): 512-515. doi: 10.3969/j.issn.1006-0111.2017.06.008
Citation: WANG Jie, HUANG Yan, ZHANG Chuan, MA Xingyuan, CHANG Wenjun, LU Yiming. Effect of 5-FU on the expression of SHP2 gene in colorectal cancer cells RKO[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(6): 512-515. doi: 10.3969/j.issn.1006-0111.2017.06.008
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氟尿嘧啶对结直肠癌RKO细胞SHP2基因表达的影响

doi: 10.3969/j.issn.1006-0111.2017.06.008
  • 1.

    第二军医大学药学院生化药学教研室, 上海 200433

  • 2.

    华东理工大学生物工程学院, 上海 200237

  • 3.

    第二军医大学热带医学与公共卫生学系环境卫生教研室, 上海 200433

基金项目: 国家自然科学基金资助项目（30500093，81274162）；国家科技部"重大新药创制"专项（2009ZX09103-690）；上海市科委生物医药领域科技支撑项目（16431904400）；上海市教委科研创新项目（14ZZ077）

Effect of 5-FU on the expression of SHP2 gene in colorectal cancer cells RKO

  • 1.

    Department of Biochemistry Pharmacy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • 2.

    College of Biological Engineering, East China University of Science and Technology, Shanghai 200237, China

  • 3.

    Department of Environmental Hygiene, School of Tropical Medicine and Public Health, Second Military Medical University, Shanghai 200433, China

  • 摘要: 目的 探究氟尿嘧啶（5-FU）对结直肠癌RKO细胞SHP2基因表达的影响。 方法 培养对数生长期的人结直肠癌细胞RKO，5-FU处理48 h后，采用免疫荧光和Western免疫印迹观察SHP2的蛋白表达水平；将能够特异性抑制SHP2表达的siRNA（siSHP2）转染RKO细胞，5-FU处理48 h，CCK8测定细胞吸光度（A）值，流式检测细胞凋亡，观察RKO对5-FU的敏感性变化及5-FU诱导细胞凋亡的影响。 结果 结直肠癌细胞RKO在5-FU处理48 h后，其细胞核中SHP2的蛋白表达水平明显增高；siSHP2转染后，RKO细胞对5-FU的敏感性降低，且降低了5-FU诱导的细胞凋亡率。 结论 5-FU可能通过影响SHP2的表达，促进RKO细胞的凋亡，发挥抑癌作用。
    Abstract: Objective To investigate the effect of 5-FU on the expression of SHP2 gene in colorectal cancer cells RKO. Methods The human colorectal cancer cell line RKO of logarithmic growth phase was cultured 48 h with 5-FU. The expression of SHP2 was observed by immunofluorescence and Western blotting; siSHP2 which specifically inhibits the expression of SHP2 was transfected into RKO cells and cultured 48 h with 5-FU. The cell absorbance (A) values were measured with CCK8 and apoptosis was determined by flow cytometry. The sensitivity of RKO to 5-FU and the effect of 5-FU on apoptosis of RKO cells were observed. Results The expression of nuclear protein of SHP2 was improved remarkably after colorectal cancer cell RKO was cultured 48 h with 5-FU. The sensitivity of RKO cells to 5-FU and the cell apoptosis rate induced by 5-FU were decreased after siSHP2 transfection. Conclusion 5-FU exerts anti-cancer activity possibly due to its promoting the apoptosis of RKO cells by influencing the expression of SHP2.
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  • 收稿日期:  2017-06-19
  • 修回日期:  2017-07-10

氟尿嘧啶对结直肠癌RKO细胞SHP2基因表达的影响

doi: 10.3969/j.issn.1006-0111.2017.06.008
  • 1. 第二军医大学药学院生化药学教研室, 上海 200433
  • 2. 华东理工大学生物工程学院, 上海 200237
  • 3. 第二军医大学热带医学与公共卫生学系环境卫生教研室, 上海 200433
    • 基金项目:  国家自然科学基金资助项目（30500093，81274162）；国家科技部"重大新药创制"专项（2009ZX09103-690）；上海市科委生物医药领域科技支撑项目（16431904400）；上海市教委科研创新项目（14ZZ077）
  • 收稿日期: 2017-06-19
  • 修回日期: 2017-07-10

摘要: 目的 探究氟尿嘧啶（5-FU）对结直肠癌RKO细胞SHP2基因表达的影响。 方法 培养对数生长期的人结直肠癌细胞RKO，5-FU处理48 h后，采用免疫荧光和Western免疫印迹观察SHP2的蛋白表达水平；将能够特异性抑制SHP2表达的siRNA（siSHP2）转染RKO细胞，5-FU处理48 h，CCK8测定细胞吸光度（A）值，流式检测细胞凋亡，观察RKO对5-FU的敏感性变化及5-FU诱导细胞凋亡的影响。 结果 结直肠癌细胞RKO在5-FU处理48 h后，其细胞核中SHP2的蛋白表达水平明显增高；siSHP2转染后，RKO细胞对5-FU的敏感性降低，且降低了5-FU诱导的细胞凋亡率。 结论 5-FU可能通过影响SHP2的表达，促进RKO细胞的凋亡，发挥抑癌作用。

English Abstract

王洁, 黄岩, 张川, 马兴元, 常文军, 陆一鸣. 氟尿嘧啶对结直肠癌RKO细胞SHP2基因表达的影响[J]. 药学实践与服务, 2017, 35(6): 512-515. doi: 10.3969/j.issn.1006-0111.2017.06.008
引用本文: 王洁, 黄岩, 张川, 马兴元, 常文军, 陆一鸣. 氟尿嘧啶对结直肠癌RKO细胞SHP2基因表达的影响[J]. 药学实践与服务, 2017, 35(6): 512-515. doi: 10.3969/j.issn.1006-0111.2017.06.008
shu
WANG Jie, HUANG Yan, ZHANG Chuan, MA Xingyuan, CHANG Wenjun, LU Yiming. Effect of 5-FU on the expression of SHP2 gene in colorectal cancer cells RKO[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(6): 512-515. doi: 10.3969/j.issn.1006-0111.2017.06.008
Citation: WANG Jie, HUANG Yan, ZHANG Chuan, MA Xingyuan, CHANG Wenjun, LU Yiming. Effect of 5-FU on the expression of SHP2 gene in colorectal cancer cells RKO[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(6): 512-515. doi: 10.3969/j.issn.1006-0111.2017.06.008
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