Protective effects of the total bakkenolides from Petasites. tricholobus on high altitude hypoxia
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摘要: 目的 探讨蜂斗菜总内酯对动物高原缺氧的保护作用。 方法 通过建立小鼠急性密闭缺氧模型、小鼠急性减压缺氧模型以及大鼠减压缺氧模型，观察蜂斗菜总内酯对实验动物存活时间及死亡率的影响，并检测动物血糖、不同脏器中糖原、三磷酸腺苷（adenosine triphosphate，ATP）、乳酸（lactic acid，LD）、乳酸脱氢酶（lactic dehydrogenase，LDH）的含量。 结果 蜂斗菜总内酯能够明显延长小鼠急性密闭缺氧的存活时间，提高存活时间增加率；能显著降低小鼠急性减压缺氧的死亡率；对于减压缺氧大鼠，能显著降低血糖含量，显著增加肝脏、骨骼肌、心肌中的糖原含量以及脑、肝脏、骨骼肌、心肌中的ATP含量，并能显著降低血浆、骨骼肌和心肌中的LD含量，降低心肌中的LDH含量。 结论 蜂斗菜总内酯对小鼠急性密闭缺氧、小鼠急性减压缺氧和大鼠减压缺氧均具有明显的保护作用，其抗高原缺氧作用与其降低血糖含量，提高脏器中糖原含量、ATP含量，降低脏器中LD及LDH含量有关。Abstract: Objective To study the protective effects of the total bakkenolides from P. tricholobus on high altitude hypoxia. Method Normobaric hypoxia model and acute hypobaric hypoxia model in mice, hypobaric hypoxia model in rats were established for this study. Survival time and survival rate of mice were recorded. The level of blood sugar and glycogen, adenosine triphosphate (ATP), lactic acid (LD), lactic dehydrogenase (LDH) were detected in different organs of rats. Results The total bakkenolides significantly prolonged the survival time of mice in normobaric hypoxia model and reduced the death rate of mice in acute hypobaric hypoxia model. The total bakkenolides suppressed blood sugar level in rats and increased the glycogen level in rat liver, skeletal muscle and myocardium. It also elevated the ATP content in rat brain, liver, skeletal muscle and myocardium. Meanwhile, the content of LD in plasma, skeletal muscle, myocardium and LDH level in myocardium were reduced. Conclusion The total bakkenolides from P. tricholobus have protective effect on normobaric hypoxia model and acute hypobaric hypoxia model in mice as well as hypobaric hypoxia model in rats. Its anti-hypoxia efficacy at high altitude may relate to the increased blood sugar, glycogen, and ATP level and reduced LD, LDH level in major organs.
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Key words:
- P. tricholobus /
- total bakkenolides /
- high altitude /
- anti-hypoxia /
- hypobaric hypoxia
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[1] 中科院中国植物志编辑委员会.中国植物志[M].北京:科学出版社,1999:94-100. [2] 国家中医药管理局《中华本草》编委会.中华本草[M].上海:上海科学技术出版社,1999:914-916. [3] 王玉亮,郭美丽,张戈,等.毛裂蜂斗菜根茎的化学成分及抗炎活性[J].第二军医大学学报,2006,27(11):1210-1213. [4] Zhang N,Guo ML,Zhang G,et al.A new neuroprotective bakkenolide from the rhizome of Petasitestricholobus[J].Chin Chem Lett,2008,19(7):841-844. [5] Wang YL,Li RP,Guo ML,et al.Bakkenolides from Petasites tricholobus and their neuroprotective effects related to antioxidant activities[J].Planta Med,2009,75(3):230-235. [6] 李余先,王燕,郭美丽.蜂斗菜的化学成分研究[J].第二军医大学学报,2010,31(7):779-781. [7] 谢曜宇,李余先,孙一鸣,等.蜂斗菜根茎中的一个新倍半萜成分及其抗缺氧活性[J].药学学报,2016,51(8):1285-1289. [8] Jiang Q,Xia YY,He JM,et al.Total bakkenolides protects neurons against cerebral ischemic injury through inhibition of NF-κB activation[J].CNS Neurol Disord Drug Targets,2014,13(5):874-884. [9] Jiang Q, Li RP,Tang Y,et al.Bakkenolide-Ⅲa protects against cerebral damage via inhibiting NF-κB activation[J].CNS Neurosci Ther,2015,21(12):943-952. [10] Netzer N,Strohl K,Faulhaber M,et al.Hypoxia-related altitude illnesses[J].J Travel Med,2013,20(4):247-255. [11] Imray C,Wright A,Subudhi A,et al.Acute mountain sickness:pathophysiology,prevention,and treatment[J].Prog Cardiovasc Dis,2010,52(6):467-484. [12] Collier DJ,Wolff CB,Hedges AM,et al.Benzolamide improves oxygenation and reduces acute mountain sickness during a high-altitude trek and has fewer side effects than acetazolamide at sea level[J].Pharmacol Res Perspect,2016,4(3):1-12. [13] Mirtschink P,KrekW.Hypoxia-driven glycolytic and fructolytic metabolic programs:Pivotal to hypertrophic heart disease[J].Biochim Biophys Acta,2016,2(11):1822-1828. [14] 马慧萍,何蕾,王昕,等.藏紫菀总黄酮对模拟高原缺氧小鼠的保护作用[J].解放军医药杂志,2016,28(6):1-4. [15] 景临林,马慧萍,樊鹏程.矮垂头菊乙醇提取物的体外自由基清除活性及其对高原缺氧小鼠的保护作用研究[J].天然产物研究与开发,2015,27:1815-1820. -
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