Effect of preoperative compound matrine injection combined with chemotherapy on lung cancer patients
-
摘要: 目的 研究术前复方苦参注射液联合化疗对肺癌患者血清学指标及肿瘤组织中凋亡分子和信号分子表达的影响。 方法 对本院确诊并拟进行手术治疗的肺癌患者，根据术前化疗方案分为复方苦参注射液联合治疗组和单纯化疗组，检测血清中肺癌标志分子含量、肺癌组织中凋亡调节分子和信号分子的mRNA含量。 结果 1血清肺癌标志分子：联合治疗组与单纯化疗组比较，前者血清中SCC、Cyfra21-1、VEGF、TGF-β1、TSGF的含量较低；2肿瘤组织中凋亡调节分子：联合治疗组与单纯化疗组比较，前者肺癌组织中Caspase-3/7、Fas/FasL、Bim的mRNA含量较高，Pim-1的mRNA含量较低；3肿瘤组织中信号分子：联合治疗组与单纯化疗组比较，前者Wnt1、β-catenin、Wnt5a、NFAT、JAK2、STAT3的mRNA含量较低。 结论 术前复方苦参注射液联合化疗能够更为有效地杀灭肺癌细胞、降低血清标志物含量、诱导癌细胞凋亡、抑制信号通路功能，是非小细胞肺癌患者术前理想的化疗方案。Abstract: Objective To study the effect of preoperative compound matrine injection combined with chemotherapy on serum index apoptotic molecules and signaling molecules expression in tumor tissues of lung cancer patients. Methods Patients with diagnosed lung cancer and planed for surgery in our hospital were selected and divided into combined marine with chemotherapy group and chemotherapy group. Then lung cancer marker molecule contents in serum and mRNA contents of apoptosis regulatory molecules, signaling molecules in tumor tissue were assayed. Results 1Serum lung cancer marker molecules:compared with chemotherapy group, lung cancer marker molecules in serum of combined therapy group SCC, Cyfra21-1, VEGF, TGF-β1, TSGF contents in serum were lower; 2mRNA contents of apoptosis regulation molecules in tumor tissues:compared with chemotherapy group, apoptosis regulation molecules mRNA contents of Caspase-3/7, Fas/FasL, Bim in tumor tissues of combined therapy group were higher, mRNA content of Pim-1 was lower; 3mRNA contents of signal molecules in tumor tissues:compared with signal molecules in tumor tissues of chemotherapy group, mRNA contents of Wnt1, β-catenin, Wnt5a, NFAT, JAK2 and STAT3 in tumor tissues of combined treatment group were lower. Conclusion Preoperative compound matrine injection combined with chemotherapy can be more effective in killing lung cancer cells, reducing serum marker levels, inducing cancer cell apoptosis, inhibiting signaling function; it's an ideal pre-surgery chemotherapy method for patients with non-small cell lung cancer.
-
Key words:
- non-small cell lung cancer (NSCLC) /
- marine /
- tumor marker /
- apoptosis /
- signal pathway
-
[1] 李铭,廖红,程恒辉. 苦参素对人肺癌A549细胞增殖及caspase3/7蛋白活性的影响[J]. 细胞与分子免疫学杂志,2013,29(5):498-499. [2] 张治业,高晓会,郭双双,等. 苦参素注射液联合NP方案治疗老年晚期NSCLC近期疗效观察[J]. 山东医药,2011,51(26):100-101. [3] 郭柳薇,高兴华,黄王莹. 肺癌患者6种血清肿瘤标志物检测结果分析[J]. 江苏医药,2014,40(1):69-71. [4] Fiala O, Pesek M, Finek J, et al. Predictive role of CEA and CYFRA 21-1 in patients with advanced-stage NSCLC treated with erlotinib[J]. Anticancer Res, 2014, 34(6):3205-3210. [5] 王敏. 血清VEGF、TGF-β1及内皮抑素在非小细胞肺癌中的表达及相关性研究[J]. 检验医学, 2013,28(8):676-680. [6] Deng B, Tan QY, Fan XQ, et al. Clinical value of assaying tumor supplied group of factor/tumor specific growth factor in patients with solitary pulmonary nodule[J]. Clin Lung Cancer, 2011, 12(3):192-196. [7] Xue Y, Wu L, Liu Y, et al. Entpd5 induces apoptosis in lung cancer cells via regulating caspase 3 expression[J]. PLoS One, 2015, 10(3):e0120046. [8] Lin YF, Lai TC, Chang CK, et al. Targeting the XIAP/caspase-7 complex selectively kills caspase-3-deficient malignancies[J]. J Clin Invest, 2013, 123(9):3861-3875. [9] 邓伟,吴一龙. 促凋亡蛋白Bim在肺癌中的研究进展[J]. 肿瘤防治研究,2014,41(11):1247-1250. [10] Siena L, Pace E, Ferraro M, et al. Gemcitabine sensitizes lung cancer cells to Fas/FasL system-mediated killing[J]. Immunology, 2014, 141(2):242-255. [11] Jin Y, Tong DY, Tang LY, et al. Expressions of Osteopontin (OPN), αvβ3 and Pim-1 associated with poor prognosis in non-small cell lung cancer (NSCLC)[J]. Chin J Cancer Res, 2012, 24(2):103-108. [12] 左慧敏,曾林祥. Wnt信号通路与非小细胞肺癌的研究进展[J]. 基础医学与临床,2015,35(2):266-269. [13] Zhang X, Lou Y, Wang H, et al. Wnt signaling regulates the stemness of lung cancer stem cells and its inhibitors exert anticancer effect on lung cancer SPC-A1 cells[J]. Med Oncol, 2015, 32(4):95. [14] Hu Y, Hong Y, Xu Y, et al. Inhibition of the JAK/STAT pathway with ruxolitinib overcomes cisplatin resistance in non-small-cell lung cancer (NSCLC)[J]. Apoptosis, 2014, 19(11):1627-1636. [15] 伊庆强,赵美,王炯轶,等. 抑制JAK2/STAT3信号通路对肺癌细胞A549中HIF-1α、VEGF和miRNA-145表达的影响[J]. 上海交通大学学报(医学版),2014,34(7):1011-1016. -
点击查看大图
计量
- 文章访问数: 3023
- HTML全文浏览量: 237
- PDF下载量: 630
- 被引次数: 0
下载:
