Effects of eleutheroside E on learning and memory ability in mice
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摘要: 目的 探讨刺五加苷E对对氯苯丙氨酸（PCPA）造模小鼠学习记忆行为以及对海马内单胺类神经递质5-羟色胺（5-HT）、5-羟吲哚乙酸（5-HIAA）、多巴胺（DA）、去甲肾上腺素（NA）的影响。 方法 Balb/c小鼠随机分为空白组，模型组，阳性药组，刺五加苷E（ELE）高、中、低剂量组。以PCPA腹腔注射建立小鼠损伤模型，给药14 d后Morris水迷宫实验测试小鼠的学习记忆能力，Elisa试剂盒测定小鼠海马组织中5-HT、5-HIAA、DA、NA的含量。 结果 行为学结果显示，模型组小鼠穿越平台次数较空白组明显降低（P<0.01），ELE高、中剂量组可明显增加小鼠穿越平台次数（P<0.01），模型组小鼠5-HT、5-HIAA较空白组显著降低（P<0.01），NA也显著降低（P<0.05）。ELE各剂量组5-HT、5-HIAA含量较模型组显著升高（P<0.01或P<0.05），ELE高、中剂量可明显改善DA、NA的含量，且与模型组相比，差异显著（P<0.01或P<0.05）。 结论 ELE提高PCPA造模小鼠学习记忆能力可能与其改善海马组织中5-HT、5-HIAA、DA和NA的含量有关。Abstract: Objective To study the influence of eleutheroside E(ELE)on the learning and memory abilities of mice induced by para-chlorophenylalanine (PCPA) and on hippocampus' monoamine neurotransmitters of serotonin(5-HT),5-hydroxindole acetic acid (5-HIAA), dopamine (DA) and norepinephrine (NA). Methods Balb/c mice were randomized into control group, model group, positive group and three treatment groups (high, moderate and low dose group). The mouse injury model was established by PCPA abdominal injection. The mice's abilities of learning and memory were detected in the Morris water maze test after 14 days of prevention medicine. The contents of 5-HT, 5-HIAA, DA and NA in mice's hippocampus were detected by Elisa kit. Results The behavior results showed that, compared with control group, the times across the platform by model group decreased significantly (P<0.01). The times across the platform by high and moderate dose groups increased significantly (P<0.01) after the administration of ELE. The contents of 5-HT and 5-HIAA of the model group were lower than those of the control group (P<0.01). The contents of NA was lower too (P<0.05). The contents of 5-HT and 5-HIAA of different dose groups were much higher than those of the model group. Conclusion The enhancement of learning and memory ability in the mouse injury model established by PCPA might be related with the improvement of the contents of 5-HT, 5-HIAA, DA, NA in mice's hippocampus by ELE.
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Key words:
- eleutheroside E /
- cognitive function /
- single amine neurotransmitter
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