Determination of doxapram hydrochloride glucose injection by HPLC
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摘要: 目的 建立盐酸多沙普仑葡萄糖注射液的高效液相色谱含量测定方法。 方法 采用COSMOSIL C18(150 mm×4.6 mm,5 μm)色谱柱,以0.82 μg/L醋酸钠溶液(用冰醋酸调pH值为4.5) -乙腈=70:30为流动相,检测波长为214 nm。 结果 盐酸多沙普仑浓度在13~91 μg/ml范围内呈良好的线性关系(r=0.999 8)。平均加样回收率为99.3%,RSD为0.81%(n=9)。 结论 此方法简便、准确、灵敏度高、专属性强。Abstract: Objective To establish an HPLC method for determination of doxapram hydrochloride glucose injection. Methods COSMOSIL C18 (150 mm×4.6 mm,5 μm) column was adopted. The mobile phase consisted of 0.82 μg/L CH3COONa (using Glacial acetic acid adjust to pH 4.5)-acetonitrile (70:30). The detection wavelength was 214 nm. Results The linearity of doxapram hydrochloride concentration was in the range of 13~91 μg/ml (r=0.999 8). The average recovery was 99.3%,RSD=0.81% (n=9). Conclusion The method was simpleaccurate and sensitive with high specificity.
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Key words:
- HPLC /
- doxapram /
- hydrochloride
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[1] 王 乐.应用拮抗药多沙普仑临床观察[J].中国医师杂志,2004,10(增刊):257. [2] 中国药典2010版.二部[S].2010:629. [3] 欧洲药典[S].第7版.1893. -
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