不同来源石斛对SH-SY5Y细胞缺血性损伤保护作用的比较

张术; 陈雅琳; 栾洁; 卞晓岚; 汪家春

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

不同来源石斛对SH-SY5Y细胞缺血性损伤保护作用的比较

张术, 陈雅琳, 栾洁, 卞晓岚, 汪家春

文章导航 > 药学实践与服务 > 2011 > 29(6): 439-441

张术, 陈雅琳, 栾洁, 卞晓岚, 汪家春. 不同来源石斛对SH-SY5Y细胞缺血性损伤保护作用的比较[J]. 药学实践与服务, 2011, 29(6): 439-441.

引用本文:

张术, 陈雅琳, 栾洁, 卞晓岚, 汪家春. 不同来源石斛对SH-SY5Y细胞缺血性损伤保护作用的比较[J]. 药学实践与服务, 2011, 29(6): 439-441.

ZHANG Shu, CHEN Ya-lin, LUAN Jie, BIAN Xiao-lan, WANG Jia-chun. Protective effect of different source of Dendrobii Caulis on ischemic injury in SH-SY5Y cells[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(6): 439-441.

Citation:

ZHANG Shu, CHEN Ya-lin, LUAN Jie, BIAN Xiao-lan, WANG Jia-chun. Protective effect of different source of Dendrobii Caulis on ischemic injury in SH-SY5Y cells[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(6): 439-441.

不同来源石斛对SH-SY5Y细胞缺血性损伤保护作用的比较

1.
海军医学研究所,上海 200433
2.
上海交通大学医学院附属瑞金医院卢湾分院,上海 200020;上海交通大学医学院附属瑞金医院,上海 200025

Protective effect of different source of Dendrobii Caulis on ischemic injury in SH-SY5Y cells

1.
Naval Medical Research Institute of CPLA, Shanghai 200433, China
2.
Luwan Branch of Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200020, China;Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China

摘要: 目的研究市售种植铁皮枫斗和组培铁皮石斛对谷氨酸损伤的SH-SY5Y细胞的保护作用。方法利用血清药理学方法制备含药血清,用SH-SY5Y细胞建立谷氨酸损伤模型,观察72 h损伤细胞增殖情况,MTT实验测定细胞活性,乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)和丙二醛(MDA)的测定研究两种来源石斛对神经细胞损伤保护作用。结果种植和组培石斛均可提高SH-SY5Y细胞谷氨酸损伤的存活率,降低培养液中LDH含量和细胞内MDA含量,提高SOD活力,其中铁皮石斛胚芽的抗氧化能力优于市售铁皮枫斗。结论石斛具有对SH-SY5Y细胞谷氨酸损伤的保护作用;组培铁皮石斛较市售铁皮枫斗保护作用更加显著,初步证明经过组培繁殖的铁皮石斛作用效果优于市售石斛属药用植物。
- 铁皮石斛 /
- 血清药理学SH-SY5Y细胞 /
- 谷氨酸 /
- 细胞损伤
Abstract: Objective To study the protective effects of planting Dendrobii caulis and tissue culture planting Dendrobium officinale Kimura et Migo on glutamate damage induced in cultured SH-SY5Y cells. Methods The sera were obtained from rats by using the serum pharmacology method, and cell damage model was established by treating SH-SY5Y cells with glutamate. the proliferation of damaged cells were observed by 72 h. MTT assay was used to test lactate dehydrogenase(LDH), malondialde-1yde(MDA)and superoxide dismutase (SOD) in the culture medium. Results Both planting and tissue culture Dendrobii caulis could promote the cell proliferation of glutamate damaged SH-SY5Y cells. They also could decrease LDH and MDA content and increase SOD level significantly. Tissue cultured Dendrobii caulis preceded planting ones in antioxidant potential. Conclusion Dendrobii caulis had protective effect on glutamate damaged SH-SY5Y cells. The protective effect of tissue culture one, while, was more distinctive than planting one, which could prove that officinal effect of tissue culture Dendrobium huoshanense was preceded to Dendrobium plants.
- Dendrobium officinale Kimura et Migo /
- serum pharmacology method SH-SY5Y cells /
- glutamate /
- cell damage

[1]	郁美娟,孟庆华,黄德音,等.石斛属植物有效成分及药理作用研究[J].中成药,2003,25(11):918.
[2]	徐叔云,卞如濂,陈修. 药理实验方法学[M]. 第3版. 北京:人民卫生出版社,2002:203.
[3]	郑永唐,贲昆龙,测定细胞存活和增殖的MTT方法的建立[J].免疫学杂志,1992,8:266.
[4]	曹定国,周汝滨,廖霞,等.rhaFGF 改构体对SH-SY5Y 细胞兴奋性氨基酸毒性损伤的作用[J].江西医学院学报 2007,47(1):18.
[5]	施红,黄玲,石斛抗衰老作用的实验研究[J].中国老年医学杂志,1994,13(2):104.
[6]	Singh J, Kaur G. Transcriptional regulation of polysialylated neural cell adhesion molecule expression by NMDA receptor activation in retinoic acid-differentiated SH-SY5Y neuroblastoma cultures[J]. Brain Res. 2007 18 (1154):8.
[7]	Jantas D, Lason W. Different mechanisms of NMDA-mediated protection against neuronal apoptosis: a stimuli-dependent effect[J]. Neurochem Res. 2009 34(11):2040.
[8]	Slemmer JE, Shacka JJ, Sweeney MI, Weber JT. Antioxidants and free radical scavengers for the treatment of stroke, traumatic brain injury and aging[J]. Curr Med Chem. 2008 15(4):404.
[9]	Corasaniti MT, Maiuolo J, Maida S, et al. Cell signaling pathways in the mechanisms of neuroprotection afforded by bergamot essential oil against NMDA-induced cell death in vitro[J]. Br J Pharmacol.2007,151(4):518.
[10]	罗璨,郭莲军.牛磺酸对大鼠急性脑缺血神经元凋亡的影响[J]. 中国药理学通报,2005,21(9):105.

[1]	姜涛, 徐卫凡, 蒋益萍, 夏天爽, 辛海量. 巴戟天丸对Aβ损伤成骨细胞的作用及基于网络药理学的机制研究 . 药学实践与服务, 2024, 42(): 1-7. doi: 10.12206/j.issn.2097-2024.202305011
[2]	徐卫凡, 徐武牧, 丁卢颖, 蒋益萍, 夏天爽, 辛海量. 巴戟天丸防治D-半乳糖损伤成骨细胞骨丢失的作用及机制研究 . 药学实践与服务, 2023, 41(3): 155-159. doi: 10.12206/j.issn.2097-2024.202202030
[3]	董雨龙, 娄成, 陈熙昀, 魏炜, 陶晨洁, 韩琴, 袁振刚. 基于网络药理学探讨通关藤抗肝细胞癌的作用机制 . 药学实践与服务, 2023, 41(10): 600-609. doi: 10.12206/j.issn.2097-2024.202212049
[4]	张倩, 郭羽晨, 邓珊珊, 章越凡, 李铁军. 基于网络药理学的益母草治疗神经损伤的潜在分子机制研究 . 药学实践与服务, 2022, 40(2): 113-119. doi: 10.12206/j.issn.1006-0111.202105106
[5]	张城, 马剑平, 沈育桦, 朱文君, 刘河龙, 邱彦. 活性多肽GRGDS对氧糖剥夺诱导PC12细胞损伤的保护作用及其机制研究 . 药学实践与服务, 2021, 39(4): 317-321, 330. doi: 10.12206/j.issn.1006-0111.202007053
[6]	韦春兰, 李文渊. 1例甲巯咪唑致全血细胞减少及肝损伤患者的药学监护 . 药学实践与服务, 2021, 39(1): 86-89. doi: 10.12206/j.issn.1006-0111.202004025
[7]	夏天爽, 刘晓燕, 蒋益萍, 李晓瑾, 王果平, 辛海量. 啤酒花经抗氧化途径减轻Aβ损伤成骨细胞作用研究 . 药学实践与服务, 2021, 39(6): 509-514. doi: 10.12206/j.issn.1006-0111.202103018
[8]	蒋益忠, 林丽娟, 辛海量, 金玉娥, 蒋益萍, 薛黎明. 四烯甲萘醌保护成骨细胞氧化损伤的作用研究 . 药学实践与服务, 2020, 38(6): 523-527. doi: 10.12206/j.issn.1006-0111.202005047
[9]	周静, 吴杲, 马福家. hsa-miRNA-30a-5p促进肺癌细胞A549增殖的机制研究 . 药学实践与服务, 2019, 37(5): 433-439. doi: 10.3969/j.issn.1006-0111.2019.05.009
[10]	满缓, 张凤, 黄豆豆, 熊筱娟, 詹勤, 廖丽娜, 李铁军, 陈万生. 升陷汤及各单味药对阿霉素致心肌细胞损伤的保护作用 . 药学实践与服务, 2019, 37(4): 304-308. doi: 10.3969/j.issn.1006-0111.2019.04.004
[11]	丁华敏, 秦春霞, 马凯源, 孙莉莉, 章越凡, 李铁军. 单核细胞移动抑制因子对大鼠创伤性颅脑损伤保护作用研究 . 药学实践与服务, 2019, 37(6): 498-502. doi: 10.3969/j.issn.1006-0111.2019.06.005
[12]	赵辉, 章越凡, 李铁军. 二氯乙酸钠对氧糖剥夺损伤的BV2细胞的保护作用及其机制研究 . 药学实践与服务, 2019, 37(2): 146-150. doi: 10.3969/j.issn.1006-0111.2019.02.009
[13]	沈启睿, 李永华, 张文杰, 王培. 同型半胱氨酸激活JNK信号通路诱导血管平滑肌细胞氧化应激的损伤研究 . 药学实践与服务, 2018, 36(6): 499-502,511. doi: 10.3969/j.issn.1006-0111.2018.06.005
[14]	任海峰, 赵英魁, 杨峰, 俞媛, 马志强. 微小RNA传递载体聚乙二醇-b-聚赖氨酸的合成及细胞毒性研究 . 药学实践与服务, 2014, 32(5): 362-365,373. doi: 10.3969/j.issn.1006-0111.2014.05.013
[15]	韩琳, 吕超, 李敏, 黄慧梅, 畅婉琳, 彭成成, 柳润辉. 麝香保心丸抗心肌细胞缺氧-复氧损伤活性成分筛选 . 药学实践与服务, 2014, 32(3): 209-211,219. doi: 10.3969/j.issn.1006-0111.2014.03.012
[16]	闫冰, 丁安伟, 张丽. 二至丸水提物对体外肝细胞损伤的保护作用 . 药学实践与服务, 2010, 28(6): 433-439,436.
[17]	叶莉, 祝德秋, 叶显撑. 荛花酚对H₂O₂和兴奋性神经递质诱导的大鼠皮层神经元细胞损伤的保护作用 . 药学实践与服务, 2010, 28(3): 189-192.
[18]	蒋国军, 路明珠, 伊佳. 原花青素对H₂O₂致PC12细胞氧化损伤作用的保护研究 . 药学实践与服务, 2009, 27(6): 414-416,420.
[19]	孙奎, 姜华. 玄参中苯丙素苷对肝细胞损伤保护作用的研究 . 药学实践与服务, 2002, (4): 234-235.
[20]	任守华. 谷氨酸钠注射液引起不良反应1例报道 . 药学实践与服务, 1997, (4): 244-244,219.

点击查看大图

计量

文章访问数: 2779
HTML全文浏览量: 251
PDF下载量: 148
被引次数: 0

不同来源石斛对SH-SY5Y细胞缺血性损伤保护作用的比较

1. 海军医学研究所,上海 200433

2. 上海交通大学医学院附属瑞金医院卢湾分院,上海 200020;上海交通大学医学院附属瑞金医院,上海 200025

收稿日期: 2011-03-16
修回日期: 2011-04-22

关键词:

铁皮石斛 /

血清药理学SH-SY5Y细胞 /

谷氨酸 /

细胞损伤

摘要: 目的研究市售种植铁皮枫斗和组培铁皮石斛对谷氨酸损伤的SH-SY5Y细胞的保护作用。方法利用血清药理学方法制备含药血清,用SH-SY5Y细胞建立谷氨酸损伤模型,观察72 h损伤细胞增殖情况,MTT实验测定细胞活性,乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)和丙二醛(MDA)的测定研究两种来源石斛对神经细胞损伤保护作用。结果种植和组培石斛均可提高SH-SY5Y细胞谷氨酸损伤的存活率,降低培养液中LDH含量和细胞内MDA含量,提高SOD活力,其中铁皮石斛胚芽的抗氧化能力优于市售铁皮枫斗。结论石斛具有对SH-SY5Y细胞谷氨酸损伤的保护作用;组培铁皮石斛较市售铁皮枫斗保护作用更加显著,初步证明经过组培繁殖的铁皮石斛作用效果优于市售石斛属药用植物。

English Abstract

Protective effect of different source of Dendrobii Caulis on ischemic injury in SH-SY5Y cells

1. Naval Medical Research Institute of CPLA, Shanghai 200433, China

2. Luwan Branch of Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200020, China;Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China

Received Date: 2011-03-16
Rev Recd Date: 2011-04-22

Keywords:

Dendrobium officinale Kimura et Migo /
serum pharmacology method SH-SY5Y cells /
glutamate /
cell damage

Abstract: Objective To study the protective effects of planting Dendrobii caulis and tissue culture planting Dendrobium officinale Kimura et Migo on glutamate damage induced in cultured SH-SY5Y cells. Methods The sera were obtained from rats by using the serum pharmacology method, and cell damage model was established by treating SH-SY5Y cells with glutamate. the proliferation of damaged cells were observed by 72 h. MTT assay was used to test lactate dehydrogenase(LDH), malondialde-1yde(MDA)and superoxide dismutase (SOD) in the culture medium. Results Both planting and tissue culture Dendrobii caulis could promote the cell proliferation of glutamate damaged SH-SY5Y cells. They also could decrease LDH and MDA content and increase SOD level significantly. Tissue cultured Dendrobii caulis preceded planting ones in antioxidant potential. Conclusion Dendrobii caulis had protective effect on glutamate damaged SH-SY5Y cells. The protective effect of tissue culture one, while, was more distinctive than planting one, which could prove that officinal effect of tissue culture Dendrobium huoshanense was preceded to Dendrobium plants.

张术, 陈雅琳, 栾洁, 卞晓岚, 汪家春. 不同来源石斛对SH-SY5Y细胞缺血性损伤保护作用的比较[J]. 药学实践与服务, 2011, 29(6): 439-441.

引用本文:

张术, 陈雅琳, 栾洁, 卞晓岚, 汪家春. 不同来源石斛对SH-SY5Y细胞缺血性损伤保护作用的比较[J]. 药学实践与服务, 2011, 29(6): 439-441.

Citation:

全文HTML

参考文献 (10)

下载: 全尺寸图片幻灯片

返回文章

用微信扫码二维码

分享至好友和朋友圈

主管、主办: 中国人民解放军海军军医大学地址: 上海市杨浦区国和路325号邮政编码: 200433

电话: （021）81871324,81871397 E-mail: yxsjzzs@163.com

网站备案号沪ICP备05003363号-1

本系统由北京仁和汇智信息技术有限公司开发

留言板

不同来源石斛对SH-SY5Y细胞缺血性损伤保护作用的比较

Protective effect of different source of Dendrobii Caulis on ischemic injury in SH-SY5Y cells

计量

出版历程

不同来源石斛对SH-SY5Y细胞缺血性损伤保护作用的比较

1. 海军医学研究所,上海 200433 2. 上海交通大学医学院附属瑞金医院卢湾分院,上海 200020;上海交通大学医学院附属瑞金医院,上海 200025

English Abstract

Protective effect of different source of Dendrobii Caulis on ischemic injury in SH-SY5Y cells

1. Naval Medical Research Institute of CPLA, Shanghai 200433, China 2. Luwan Branch of Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200020, China;Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China

全文HTML

目录

1. 海军医学研究所,上海 200433

2. 上海交通大学医学院附属瑞金医院卢湾分院,上海 200020;上海交通大学医学院附属瑞金医院,上海 200025

1. Naval Medical Research Institute of CPLA, Shanghai 200433, China

2. Luwan Branch of Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200020, China;Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China