葡萄籽原花青素对血管性痴呆大鼠空间学习记忆能力的影响

周恒; 林焕冰; 卓烨烨; 毕炳添; 李国熊; 徐江平

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葡萄籽原花青素对血管性痴呆大鼠空间学习记忆能力的影响

周恒, 林焕冰, 卓烨烨, 毕炳添, 李国熊, 徐江平

文章导航 > 药学实践与服务 > 2011 > 29(3): 208-211

周恒, 林焕冰, 卓烨烨, 毕炳添, 李国熊, 徐江平. 葡萄籽原花青素对血管性痴呆大鼠空间学习记忆能力的影响[J]. 药学实践与服务, 2011, 29(3): 208-211.

引用本文:

周恒, 林焕冰, 卓烨烨, 毕炳添, 李国熊, 徐江平. 葡萄籽原花青素对血管性痴呆大鼠空间学习记忆能力的影响[J]. 药学实践与服务, 2011, 29(3): 208-211.

ZHOU Heng, LIN Huan-bing, ZHUO Ye-ye, Bi Bing-tian, Li Guo-xiong, XU Jiang-ping. Effects of grape seed procyanidins on spatial learning and memory in vascular dementia model of rats[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(3): 208-211.

Citation:

ZHOU Heng, LIN Huan-bing, ZHUO Ye-ye, Bi Bing-tian, Li Guo-xiong, XU Jiang-ping. Effects of grape seed procyanidins on spatial learning and memory in vascular dementia model of rats[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(3): 208-211.

葡萄籽原花青素对血管性痴呆大鼠空间学习记忆能力的影响

1.
南方医科大学药学院神经药理学科,广东广州 510515
2.
广东省食品药品监督管理局审评认证中心,广东广州 510080

Effects of grape seed procyanidins on spatial learning and memory in vascular dementia model of rats

1.
Department of Neuropsychopharmacology, School of Pharmacy, Southern Medical University, Guangzhou 510515, China
2.
Center for evaluation & certification, Guangdong food and drug administration, Guangzhou 510080, China

摘要: 目的观察葡萄籽原花青素(GSP)对血管性痴呆(VD)大鼠空间学习记忆能力的影响,探讨其可能的作用机制。方法采用改良Pulsinelli四血管阻塞法建立VD大鼠模型,造模成功后,每天灌胃给药,Wistar大鼠分为假手术组,模型组,原花青素组(50 mg/kg和150 mg/kg),银杏叶片24 mg/kg。Morris水迷宫检测VD大鼠空间学习记忆能力;比色法检测脑组织超氧化物歧化酶(SOD)活性、总抗氧化能力(T-AOC)、谷胱甘肽(GSH)、丙二醛(MDA)含量;实时荧光定量PCR(Real-time PCR)法检测海马Bax、Bcl-2 mRNA的表达。结果与模型组比较,原花青素可明显改善VD大鼠空间学习记忆能力;提高脑组织SOD活性、GSH含量和总抗氧化能力,降低MDA含量;提高海马Bcl-2/Bax mRNA比值。结论原花青素可改善VD大鼠学习记忆功能,其机制可能与提高机体抗氧化能力、抑制海马神经元凋亡有关。
- 原花青素 /
- 血管性痴呆 /
- 氧化应激 /
- 凋亡
Abstract: Objective To investigate the effects and possible mechanisms of procyanidins, a polyphenol extracted from grape seeds, on spatial learning and memory in rats model of vascular dementia(VD). Methods Modified Pulsinelli's 4-vessel occlusion (4-VO) method was used for replicating vascular dementia in rats. The male Wistar rats were divided equally into 5 experimental groups: in one the rats were subjected to sham-operated, and in the other four the rats was subjected to the 4-VO surgery followed by oral administration of vehicle, Ginkgo leaf extract(24 mg/kg), procyanidins(50 or 150 mg/kg) respectively. The spatial learning and memory performance was recorded as the acquisition trial and probe trial in the Morris water maze. The levels of malondialdehyde(MDA), glutathione(GSH), total antioxidant capacity(T-AOC) and the activity of superoxidedismutase(SOD) in the brain tissue were determined by colorimetric Assay Kit. The gene expression of Bax and Bcl-2 in the rat hippocampus was detected by real-time PCR. Results The vascular dementia rats treated with procyanidins showed decreased escaped latency during acquisition training, especially at the dose of 150 mg/kg, procyanidins also increased exploration time and frequency of crossing in the target quadrant during probe trial. The administration of procyanidins induced increase in GSH, T-AOC contents and SOD activities with lowering MDA levels in the brain. In addition, procyanidins upregulated the Bcl-2/Bax ratio of mRNA expression in the rat hippocampus. Conclusion Procyanidins could have potentials for improving the spatial cognition with VD,which was possibly due to the antioxidative and anti-apoptotic effects of polyphenol.
- procyanidins /
- vascular dementia /
- oxidative stress /
- apoptosis

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葡萄籽原花青素对血管性痴呆大鼠空间学习记忆能力的影响

1. 南方医科大学药学院神经药理学科,广东广州 510515

2. 广东省食品药品监督管理局审评认证中心,广东广州 510080

收稿日期: 2011-01-07
修回日期: 2011-03-13

关键词:

摘要: 目的观察葡萄籽原花青素(GSP)对血管性痴呆(VD)大鼠空间学习记忆能力的影响,探讨其可能的作用机制。方法采用改良Pulsinelli四血管阻塞法建立VD大鼠模型,造模成功后,每天灌胃给药,Wistar大鼠分为假手术组,模型组,原花青素组(50 mg/kg和150 mg/kg),银杏叶片24 mg/kg。Morris水迷宫检测VD大鼠空间学习记忆能力;比色法检测脑组织超氧化物歧化酶(SOD)活性、总抗氧化能力(T-AOC)、谷胱甘肽(GSH)、丙二醛(MDA)含量;实时荧光定量PCR(Real-time PCR)法检测海马Bax、Bcl-2 mRNA的表达。结果与模型组比较,原花青素可明显改善VD大鼠空间学习记忆能力;提高脑组织SOD活性、GSH含量和总抗氧化能力,降低MDA含量;提高海马Bcl-2/Bax mRNA比值。结论原花青素可改善VD大鼠学习记忆功能,其机制可能与提高机体抗氧化能力、抑制海马神经元凋亡有关。

English Abstract

Effects of grape seed procyanidins on spatial learning and memory in vascular dementia model of rats

1. Department of Neuropsychopharmacology, School of Pharmacy, Southern Medical University, Guangzhou 510515, China

2. Center for evaluation & certification, Guangdong food and drug administration, Guangzhou 510080, China

Received Date: 2011-01-07
Rev Recd Date: 2011-03-13

Keywords:

Abstract: Objective To investigate the effects and possible mechanisms of procyanidins, a polyphenol extracted from grape seeds, on spatial learning and memory in rats model of vascular dementia(VD). Methods Modified Pulsinelli's 4-vessel occlusion (4-VO) method was used for replicating vascular dementia in rats. The male Wistar rats were divided equally into 5 experimental groups: in one the rats were subjected to sham-operated, and in the other four the rats was subjected to the 4-VO surgery followed by oral administration of vehicle, Ginkgo leaf extract(24 mg/kg), procyanidins(50 or 150 mg/kg) respectively. The spatial learning and memory performance was recorded as the acquisition trial and probe trial in the Morris water maze. The levels of malondialdehyde(MDA), glutathione(GSH), total antioxidant capacity(T-AOC) and the activity of superoxidedismutase(SOD) in the brain tissue were determined by colorimetric Assay Kit. The gene expression of Bax and Bcl-2 in the rat hippocampus was detected by real-time PCR. Results The vascular dementia rats treated with procyanidins showed decreased escaped latency during acquisition training, especially at the dose of 150 mg/kg, procyanidins also increased exploration time and frequency of crossing in the target quadrant during probe trial. The administration of procyanidins induced increase in GSH, T-AOC contents and SOD activities with lowering MDA levels in the brain. In addition, procyanidins upregulated the Bcl-2/Bax ratio of mRNA expression in the rat hippocampus. Conclusion Procyanidins could have potentials for improving the spatial cognition with VD,which was possibly due to the antioxidative and anti-apoptotic effects of polyphenol.

周恒, 林焕冰, 卓烨烨, 毕炳添, 李国熊, 徐江平. 葡萄籽原花青素对血管性痴呆大鼠空间学习记忆能力的影响[J]. 药学实践与服务, 2011, 29(3): 208-211.

引用本文:

周恒, 林焕冰, 卓烨烨, 毕炳添, 李国熊, 徐江平. 葡萄籽原花青素对血管性痴呆大鼠空间学习记忆能力的影响[J]. 药学实践与服务, 2011, 29(3): 208-211.

Citation:

ZHOU Heng, LIN Huan-bing, ZHUO Ye-ye, Bi Bing-tian, Li Guo-xiong, XU Jiang-ping. Effects of grape seed procyanidins on spatial learning and memory in vascular dementia model of rats[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(3): 208-211.

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葡萄籽原花青素对血管性痴呆大鼠空间学习记忆能力的影响

Effects of grape seed procyanidins on spatial learning and memory in vascular dementia model of rats

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葡萄籽原花青素对血管性痴呆大鼠空间学习记忆能力的影响

1. 南方医科大学药学院神经药理学科,广东 广州 510515 2. 广东省食品药品监督管理局审评认证中心,广东 广州 510080

English Abstract

Effects of grape seed procyanidins on spatial learning and memory in vascular dementia model of rats

1. Department of Neuropsychopharmacology, School of Pharmacy, Southern Medical University, Guangzhou 510515, China 2. Center for evaluation & certification, Guangdong food and drug administration, Guangzhou 510080, China

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1. 南方医科大学药学院神经药理学科,广东广州 510515

2. 广东省食品药品监督管理局审评认证中心,广东广州 510080

1. Department of Neuropsychopharmacology, School of Pharmacy, Southern Medical University, Guangzhou 510515, China

2. Center for evaluation & certification, Guangdong food and drug administration, Guangzhou 510080, China