留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码
x 关闭 永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

聚氨基酯作为基因载体的研究进展

王江峰,  鲁莹,  钟延强

downloadPDF
文章导航 > 药学实践与服务  > 2011 >  29(3): 165-168
王江峰, 鲁莹, 钟延强. 聚氨基酯作为基因载体的研究进展[J]. 药学实践与服务, 2011, 29(3): 165-168.
引用本文: 王江峰, 鲁莹, 钟延强. 聚氨基酯作为基因载体的研究进展[J]. 药学实践与服务, 2011, 29(3): 165-168.
shu
WANG Jiang-feng, LU Ying, ZHONG Yan-qiang. Research on poly amino ester as the gene vector[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(3): 165-168.
Citation: WANG Jiang-feng, LU Ying, ZHONG Yan-qiang. Research on poly amino ester as the gene vector[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(3): 165-168.
shu

聚氨基酯作为基因载体的研究进展

  • 第二军医大学药剂学教研室,上海 200433

Research on poly amino ester as the gene vector

  • Department of pharmaceutical, Second Military Medical University, Shanghai 200433, China

  • 摘要: 随着基因治疗研究的不断深入,寻求一种高效、安全、靶向表达的载体已成为了基因治疗的核心问题之一。聚氨基酯(poly amino ester,PAE)作为一种新型的阳离子聚合物基因载体,具有原料廉价、合成简单、结构多样、可降解、细胞毒性低和转染效率高等优点,已越来越受到人们的关注。本文将从聚氨基酯的合成途径、理化性质、聚氨基酯/DNA纳米粒的制备及聚氨基酯/DNA纳米粒转染效率和影响因素等方面详细阐述了近年来聚氨基酯作为基因载体的研究进展。
    Abstract: With the deepening research of gene therapy, to find an efficient, safe, targeted expression gene vector had become one of the core issues of gene therapy. Poly amino ester (PAE) as a new type of cationic polymer gene carriers, with cheap raw materials, simple synthesis, diversity structure, biodegradability, low toxicity and high efficiency transfection, which had attracted lots of researchers' focus. Recent research of PAE as a gene vector from the poly amino ester synthesis pathway, physical and chemical properties, properties of poly amino ester / DNA nano-particles and transfection efficiency of polyamino ester/DNA nano-particles were summarized in this paper.
  • [1] Sun JY, Anand-Jawa V, Chatterjee S, et al. Immune responses to adeno-associated virus and its recombinant vectors[J]. Gene Ther, 2003,10: 964.
    [2] Gore ME. Adverse effects of gene therapy: gene therapy can cause leukaemia: no shock, mild horror but a probe[J]. Gene Ther, 2003, 10: 4.
    [3] Boussif O, Lezoualc'h F, Zanta, et al. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine[J]. Proc Natl Acad Sci, 1995, 92: 7297.
    [4] Luo D, Saltzman WM. Synthetic DNA delivery systems[J]. NatBiotechnol, 2000, 18 (1):33.
    [5] Lemkine GF, Demeneix BA. Polyethylenimines for in vivo gene delivery[J]. Curr Opin Mol Ther, 2001, 3: 178.
    [6] Danusso F, Ferruti P. Synthesis of tertiary amine polymers[J]. Polymer, 1970, 11: 88.
    [7] Lynn DM, Robert Langer. Degradable poly(β-amino esters): synthesis, characterization, and self-assembly with plasmid DNA[J]. Am Chem Soc, 2000, 122:10761.
    [8] Lynn DM, Anderson DG, Putnam D, et al. Accelerated discovery of synthetic transfection vectors: parallel synthesis and screening of a degradable polymer library[J]. Am Chem Soc, 2001, 123: 8155.
    [9] Akin Akinc, Anderson DG, Lynn DM, et al. Synthesis of poly(β-amino ester)s optimized for highly effective gene delivery[J]. Bioconjugate Chem, 2003, 14: 979.
    [10] Anderson DG, Lynn DM, and Robert Langer. Semi-automated synthesis and screening of a large library of degradable cationic polymers for gene delivery[J]. Angew Chem Int Ed, 2003, 42: 3153 .
    [11] Zhong Zhiyuan , Song Yan , Johan FJ, et al. A versatile family of degradable non-viral gene carriers based on hyperbranched poly(ester amine)s[J]. Journal of Controlled Release, 2005,109: 317.
    [12] Tae-il Kim, Hyo Jung Seo, Joon Sig Choi, et al. Synthesis of biodegradable cross-linked poly(β-amino ester) for gene delivery and its modification, inducing enhanced transfection efficiency and stepwise degradation[J]. Bioconjugate Chem. 2005, 16:1140.
    [13] Hyun JK, Min SK, Joon SC, et al. Synthesis and characterization of poly (amino ester) for slow biodegradable gene delivery vector[J]. Bioorganic & Medicinal Chemistry, 2007, 15: 1708.
    [14] Liu G, Molas M, Grossmann GA, et al. Biological properties of poly-L-lysine-DNA complexes generated by cooperative binding of the polycation[J]. Biol Chem 2001, 276: 34379.
    [15] Ogris M, Steinlein P, Carotta S, et al. DNA/polyethylenimine transfection particles: influence of ligands, polymer size, and PEGylation on internalization and gene expression[J]. AAPS PharmSci. 2001, 3: 21.
    [16] Anderson DG, Akinc A, Hossain N, et al. Structure/property studies of polymeric gene delivery using a library of poly(beta-amino esters) [J]. Mol Ther, 2005, 11: 426.
    [17] Guo W, Lee RJ. Efficient gene delivery via non-covalent complexes of folic acid and polyethylenimine[J]. Controlled Release 2001, 77: 131.
    [18] Green J, Shi J, Chiu E, et al. Biodegradable polymeric vectors for gene delivery to human endothelial cells[J]. Bioconjugate Chem, 2006, 1: 1162.
    [19] 韦卫中.阳离子聚合物纳米基因载体的研究进展[J].医学研究生学报,2004,17(4):335.
    [20] Akinc A, Langer R. Measuring the pH environment of DNA delivered using nonviral vectors: implications for lysosomal trafficking[J]. Biotechnol Bioeng, 2002, 78:503.
    [21] Schaffer DV, Fidelman NA, Dan N, et al. Vector unpacking as a potential barrier for receptor-mediated polyplex gene delivery[J]. Biotechnol Bioeng, 2000, 67: 598.
    [22] Choosakoonkriang S, Lobo BA, Koe GS, et al. Biophysical characterization of PEI/DNA complexes[J]. Pharm Sci, 2003, 92:1710.
    [23] Zugates GT, Nathan CT, Andreas Zumbuehl, et al. Gene delivery properties of end-modified poly(beta-amino ester)s[J]. Bioconjugate Chem. 2007, 18: 1887.
    [24] Zugates G, Anderson D, Little S, et al. Synthesis of poly(beta amino ester)s with thiol-reactive side chains for DNA delivery[J]. Am Chem Soc, 2006, 128: 12726.
    [25] Green JJ, Chiu E, Leshchiner ES, et al. Electrostatic ligand coatings of nanoparticles enable ligand-specific gene delivery to human primary cells[J]. Nano Lett, 2007, 7: 874.
  • [1] 卞康晴, 郭灵怡, 迟文雅, 俞媛.  聚合物囊泡的稳定性及H+透膜特性考察 . 药学实践与服务, 2024, 42(1): 12-17. doi: 10.12206/j.issn.2097-2024.202309010
    [2] 李婷, 时扣荣, 李智, 王子玉, 曹爱霖, 钱皎.  达比加群酯治疗老年非瓣膜性心房颤动的有效性和安全性评价 . 药学实践与服务, 2023, 41(9): 557-560. doi: 10.12206/j.issn.2097-2024.202207029
    [3] 林昕, 张佳良, 周欣, 宋洪涛.  他克莫司阳离子微乳凝胶的兔眼刺激性研究及房水药动学研究 . 药学实践与服务, 2023, 41(3): 177-181. doi: 10.12206/j.issn.2097-2024.202104055
    [4] 田硕, 厉建中.  ZNF24基因过表达慢病毒载体的构建及其在人结直肠癌HCT116细胞中的表达 . 药学实践与服务, 2023, 41(4): 222-226. doi: 10.12206/j.issn.2097-2024.202204040
    [5] 林昕, 张佳良, 宋洪涛.  他克莫司阳离子微乳原位凝胶的制备及体外释放研究 . 药学实践与服务, 2020, 38(4): 307-311, 317. doi: 10.12206/j.issn.1006-0111.201911075
    [6] 刘丹, 张军东, 廉云飞, 房秋雨, 李娟.  核-壳结构的脂质-聚合物杂化纳米粒的研究进展 . 药学实践与服务, 2018, 36(1): 13-17. doi: 10.3969/j.issn.1006-0111.2018.01.003
    [7] 王欢, 马志强, 杨峰.  用于肿瘤治疗的小分子干扰RNA非病毒载体研究进展 . 药学实践与服务, 2015, 33(6): 498-501. doi: 10.3969/j.issn.1006-0111.2015.06.005
    [8] 解方园, 俞媛, 耿雯倩, 钟延强.  双配体修饰聚合物泡囊的制备及细胞摄取 . 药学实践与服务, 2015, 33(1): 44-48. doi: 10.3969/j.issn.1006-0111.2015.01.011
    [9] 刘敏, 张海, 蔡亚梅, 陈啸飞, 朱臻宇, 柴逸峰.  附子总生物碱提取物中3个双酯型和3个单酯型乌头碱成分的含量测定 . 药学实践与服务, 2013, 31(3): 181-184. doi: 10.3969/j.issn.1006-0111.2013.03.005
    [10] 吴红媛, 厉建中, 张俊平.  核糖体蛋白RPS3a腺病毒载体的构建和体外表达 . 药学实践与服务, 2013, 31(5): 347-350. doi: 10.3969/j.issn.1006-0111.2013.05.007
    [11] 吴韫韬, 张依依.  聚合物胶束作为药物载体及其在肿瘤靶向方面的研究进展 . 药学实践与服务, 2013, 31(2): 86-89,115. doi: 10.3969/j.issn.1006-0111.2013.02.002
    [12] 郭志鑫, 孙会敏, 杨锐, 高志峰, 王华.  药用辅料聚山梨酯80(吐温80)质量状况分析 . 药学实践与服务, 2012, 30(6): 459-461. doi: 10.3969/j.issn.1006-0111.2012.06.019
    [13] 李晏.  作为基因输送载体的壳聚糖衍生物研究进展 . 药学实践与服务, 2011, 29(1): 8-10,61.
    [14] 孙霁, 陈琰, 鲁莹, 钟延强.  阴离子脂质体－阳离子脂质体复合物介导的基因转染 . 药学实践与服务, 2011, 29(4): 265-268.
    [15] 韩保民, 胡永胜.  碘附(聚维酮碘)名称辩析 . 药学实践与服务, 2009, 27(5): 388-389.
    [16] 蒋晔, 徐智儒, 田孟军.  非水酸碱电位滴定法测定阿德福韦酯原料药含量 . 药学实践与服务, 2004, (5): 291-293.
    [17] 张立超, 胡晋红.  聚合物水分散体包衣技术的进展 . 药学实践与服务, 1999, (3): 144-148.
    [18] 李玉平.  建议减少使用或停止使用含非那西丁、氨基比林成份的解热镇痛药 . 药学实践与服务, 1997, (4): 213-213.
    [19] 郭涛.  释放肽和蛋白质的聚合物 . 药学实践与服务, 1995, (2): 86-86.
    [20] 陈士景, 汤森节, 周全.  喷雾干燥的微型包囊工艺和不同聚合物包料对其的影响 . 药学实践与服务, 1993, (2): 131-133.
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  2284
  • HTML全文浏览量:  187
  • PDF下载量:  257
  • 被引次数: 0
出版历程
  • 收稿日期:  2010-08-02
  • 修回日期:  2010-10-12

聚氨基酯作为基因载体的研究进展

  • 第二军医大学药剂学教研室,上海 200433
  • 收稿日期: 2010-08-02
  • 修回日期: 2010-10-12

摘要: 随着基因治疗研究的不断深入,寻求一种高效、安全、靶向表达的载体已成为了基因治疗的核心问题之一。聚氨基酯(poly amino ester,PAE)作为一种新型的阳离子聚合物基因载体,具有原料廉价、合成简单、结构多样、可降解、细胞毒性低和转染效率高等优点,已越来越受到人们的关注。本文将从聚氨基酯的合成途径、理化性质、聚氨基酯/DNA纳米粒的制备及聚氨基酯/DNA纳米粒转染效率和影响因素等方面详细阐述了近年来聚氨基酯作为基因载体的研究进展。

English Abstract

王江峰, 鲁莹, 钟延强. 聚氨基酯作为基因载体的研究进展[J]. 药学实践与服务, 2011, 29(3): 165-168.
引用本文: 王江峰, 鲁莹, 钟延强. 聚氨基酯作为基因载体的研究进展[J]. 药学实践与服务, 2011, 29(3): 165-168.
shu
WANG Jiang-feng, LU Ying, ZHONG Yan-qiang. Research on poly amino ester as the gene vector[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(3): 165-168.
Citation: WANG Jiang-feng, LU Ying, ZHONG Yan-qiang. Research on poly amino ester as the gene vector[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(3): 165-168.
shu

    全文HTML

参考文献 (25)

目录

    /

    返回文章
    返回

    版权所有：《药学实践与服务》编辑委员会

    主管、主办: 中国人民解放军海军军医大学地址: 上海市杨浦区国和路325号邮政编码: 200433

    电话: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    本系统由 北京仁和汇智信息技术有限公司 开发    百度统计