丹参酮ⅡA的荧光分析法研究
Determination of Tanshinone IIA by Fluorescence Spectrophotometry
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摘要: 目的 研究中药材丹参有效成分丹参酮ⅡA的荧光光谱特性及其分析方法。 方法 分别研究浓度、酸度对丹参酮ⅡA荧光强度的影响,并研究丹参酮ⅡA的荧光稳定性。 结果 丹参酮ⅡA在酸性(pH=2)环境具有很好的荧光特性,在紫外照射下荧光稳定性良好。 结论 本方法简便、灵敏,能够满足药物定性、定量分析的要求。Abstract: Objective To study the fluorescence properties and analysis method of Tanshinone IIA. Methods Influencial factors including acidic media, the concentrations of substrate on the fluorescence intensity were studied. The stability of fluorescence of Tanshinone IIA was studied. Results Tanshinone IIA had good fluorescent characteristic in pH 2, which had an emission of fluorescence steadily under ultra-vioket radiation. Conclusion The method is simple, sensitive and could be suitable for the qualitative and quantitative analysis.
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Key words:
- Tanshin /
- Tanshinone IIA /
- Fluorescence Spectrophotometry
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