五味子与黄芪多糖协同保护对乙酰氨基酚致小鼠急性肝损伤

燕菲; 张巧艳; 张宏; 韩婷; 焦磊; 秦路平

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

五味子与黄芪多糖协同保护对乙酰氨基酚致小鼠急性肝损伤

燕菲, 张巧艳, 张宏, 韩婷, 焦磊, 秦路平

文章导航 > 药学实践与服务 > 2009 > 27(5): 340-344

燕菲, 张巧艳, 张宏, 韩婷, 焦磊, 秦路平. 五味子与黄芪多糖协同保护对乙酰氨基酚致小鼠急性肝损伤[J]. 药学实践与服务, 2009, 27(5): 340-344.

引用本文:

燕菲, 张巧艳, 张宏, 韩婷, 焦磊, 秦路平. 五味子与黄芪多糖协同保护对乙酰氨基酚致小鼠急性肝损伤[J]. 药学实践与服务, 2009, 27(5): 340-344.

YAN Fei, ZHANG Qiao-yan, ZHANG Hong, HAN Ting, JIAO Lei, QIN Lu-ping. Synergistic protective effect of Fructus Schisandrae chinensis with Astragalus polysaccharides on paracetamol-induced acute liver injury in mice[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(5): 340-344.

Citation:

YAN Fei, ZHANG Qiao-yan, ZHANG Hong, HAN Ting, JIAO Lei, QIN Lu-ping. Synergistic protective effect of Fructus Schisandrae chinensis with Astragalus polysaccharides on paracetamol-induced acute liver injury in mice[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(5): 340-344.

五味子与黄芪多糖协同保护对乙酰氨基酚致小鼠急性肝损伤

第二军医大学药学院生药学教研室,上海200433

Synergistic protective effect of Fructus Schisandrae chinensis with Astragalus polysaccharides on paracetamol-induced acute liver injury in mice

Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

摘要: 目的:研究五味子提取物和黄芪多糖配伍后对对乙酰氨基酚引起小鼠急性肝损伤的协同保护作用及其机制。方法:以对乙酰氨基酚500 mg/kg腹腔注射给药造成小鼠急性肝损伤模型,通过测定小鼠血清丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)的活性、肝组织还原性谷胱甘肽(GSH)和丙二醛(MDA)含量及观察肝组织病理学改变,以评价五味子提取物、黄芪多糖及其组合物对小鼠肝损伤的保护作用。结果:五味子提取物(270 mg/kg)和黄芪多糖(900 mg/kg)单独给药对对乙酰氨基酚肝损伤小鼠的血清ALT、AST和肝组织GSH和MDA没有显著性影响。30、90和270 mg/kg五味子提取物分别与100、300和900 mg/kg黄芪多糖配伍,可使对乙酰氨基酚肝损伤小鼠血清ALT、AST活性及肝组织MDA含量显著降低(P<0.05或P<0.01),肝组织GSH含量显著提高(P<0.05或P<0.01),肝组织细胞的病变程度得到明显改善。在高剂量配伍下,各指标的五味子提取物和黄芪多糖间相互作用指数CDI均小于0.7。结论:五味子提取物和黄芪多糖通过协同提高对乙酰氨基酚致肝损伤小鼠肝脏的还原性谷胱甘肽和抗氧化水平,降低血清ALT和AST水平,减轻肝组织细胞的损伤。
- 五味子 /
- 黄芪多糖 /
- 对乙酰氨基酚 /
- 肝损伤
Abstract: Objective : To study the synergistic protective effect and mechanism of extracts from Fructus Schisandrae chinensis(EFS) with astragalus polysaccharides(APS) on paracetamol-induced liver injury in mice. Methods :Mice liver injury was induced by peritoneal injection paracetamol physiologic saline solution at a dose of 500 mg/kg.The activities of serum alanine aminotransferase(ALT) and asparate aminotransferase(AST),the contents of malondialdehyde(MDA) and glutathione(GSH) in the liver tissue,and the liver histopathological changes were observed to evaluate the effects of EFS,APS and their combination on liver injury. Results : The EFS(270 mg/kg) or APS(900 mg/kg) alone did not produce any effect on liver injury induced by paracetamol in mice.The combination of EFS at dose of 30、90 and 270 mg/kg respectively with APS at dose of 100、300 and 900 mg/kg significantly decreased the activities of ALT and AST in serum and MAD level in liver tissue(P<0.05 or P<0.01),increased the GSH content in liver tissue(P<0.05 or P<0.01),and improved liver histopathological changes.The coefficients drug interaction(CDI) of related parameters between EFS(270 mg/kg) and APS(900 mg/kg) were all less than 0.7. Conclusion :The combination of EFS and APS reduced the ALT and AST level in serum,improved the liver histopathological changes of liver injury mice through synergistically increasing GSH content and antioxidant ability of liver.
- Schisandra chinensis /
- astragalus polysaccharides /
- paracetamol /
- liver injury

[1]	于德勋, 黄心慧, 朱可涵, 黄婷, 周婷婷, 高建义. UHPLC法测定辐射后对乙酰氨基酚缓释片含量 . 药学实践与服务, 2022, 40(6): 550-552, 562. doi: 10.12206/j.issn.2097-2024.202109100
[2]	韦春兰, 李文渊. 1例甲巯咪唑致全血细胞减少及肝损伤患者的药学监护 . 药学实践与服务, 2021, 39(1): 86-89. doi: 10.12206/j.issn.1006-0111.202004025
[3]	张津, 张文静. 新型冠状病毒肺炎患者肝损伤的原因及保肝药物治疗 . 药学实践与服务, 2020, 38(6): 481-484. doi: 10.12206/j.issn.1006-0111.202003055
[4]	周婷, 蔡建, 段晶晶, 刘芬莲, 胡芳宝, 陆赛花. 丙戊酸钠用药差错致肝损伤:病例分析 . 药学实践与服务, 2019, 37(5): 466-469. doi: 10.3969/j.issn.1006-0111.2019.05.016
[5]	王玉鹏, 鲍婕. 异烟肼致肝损伤发病机制的研究进展 . 药学实践与服务, 2019, 37(4): 289-293. doi: 10.3969/j.issn.1006-0111.2019.04.001
[6]	王妍, 舒鹏, 沈丽, 丁明, 胡振林, 吴明江. 五味子提取工艺优化及乙醇提取物的皮肤细胞抗氧化作用研究 . 药学实践与服务, 2019, 37(3): 231-236. doi: 10.3969/j.issn.1006-0111.2019.03.008
[7]	蒋益萍, 薛黎明, 辛海量, 张巧艳, 秦路平. AB-8大孔树脂纯化五味子木脂素工艺研究 . 药学实践与服务, 2017, 35(6): 520-525. doi: 10.3969/j.issn.1006-0111.2017.06.010
[8]	唐颖, 梁琳, 郭美丽. 青葙总皂苷对肝损伤保护作用的研究 . 药学实践与服务, 2016, 34(3): 201-205,282. doi: 10.3969/j.issn.1006-0111.2016.03.003
[9]	吕建峰, 刘华, 叶爱琴, 刘君, 丁荣杰. 异甘草酸镁预防奥沙利铂肝损伤的临床观察 . 药学实践与服务, 2015, 33(2): 171-172. doi: 10.3969/j.issn.1006-0111.2015.02.021
[10]	夏爱军, 张琪, 张云波, 梁园, 唐哲, 周宁宁. 舒肝安乐宁浸膏对小鼠急性酒精性肝损伤保护作用的研究 . 药学实践与服务, 2011, 29(5): 366-368.
[11]	夏爱军, 张琪, 梁园. 舒肝安乐宁浸膏对小鼠急性肝损伤的保护作用 . 药学实践与服务, 2010, 28(5): 352-353,380.
[12]	刘桂萍, 秦峰, 贾淑琴. 康必得服用过量致严重精神异常1例 . 药学实践与服务, 2009, 27(4): 316-316.
[13]	于海瑶, 肖秋生, 洪建飞, 王艳萍, 陈正爱, 刘莹, 王贺雷, 郭玉香. 黄芪有效部位不同提取分离方法的比较 . 药学实践与服务, 2008, (1): 20-22,47.
[14]	孙宁玲, 汪涛, 袁本利, 李慧梁, 陆国才, 张卫东, 张川, 袁伯俊. 脱氢卡维汀对四氯化碳致小鼠急性肝损伤的保护作用 . 药学实践与服务, 2008, (1): 23-27,50.
[15]	柳润辉, 陈忠梁, 李铁军, 刘晓华, 徐瑞林, 徐希科, 张川, 张卫东. 胆宁片对实验性急慢性肝损伤的保护作用 . 药学实践与服务, 2007, (3): 147-149.
[16]	刘萌, 李志梅. 高效液相色谱法测定复方氨酚葡锌片中对乙酰氨基酚的含量 . 药学实践与服务, 2004, (3): 167-168.
[17]	黄燕萍. 差示分光光度法测定复方感冒灵片中对乙酰氨基酚的含量 . 药学实践与服务, 2001, (1): 26-26.
[18]	陈坚, 方维军, 张灯玲, 丁宗政, 陈睿. 反相高效液相色谱法测定对乙酰氨基酚血药浓度 . 药学实践与服务, 1998, (5): 296-298.
[19]	曲奎山, 吴春福. 病毒清对四氯化碳致小鼠肝损伤的保护作用 . 药学实践与服务, 1997, (2): 79-81.
[20]	王锦, 杨丽彬, 韩家荣, 李忠义, 于占荣. 紫外分光光度法测定对乙酰氨基酚泡腾片含量 . 药学实践与服务, 1995, (3): 181-183.

点击查看大图

计量

文章访问数: 2290
HTML全文浏览量: 278
PDF下载量: 862
被引次数: 0

五味子与黄芪多糖协同保护对乙酰氨基酚致小鼠急性肝损伤

第二军医大学药学院生药学教研室,上海200433

收稿日期: 2009-01-06

关键词:

五味子 /

黄芪多糖 /

对乙酰氨基酚 /

肝损伤

摘要: 目的:研究五味子提取物和黄芪多糖配伍后对对乙酰氨基酚引起小鼠急性肝损伤的协同保护作用及其机制。方法:以对乙酰氨基酚500 mg/kg腹腔注射给药造成小鼠急性肝损伤模型,通过测定小鼠血清丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)的活性、肝组织还原性谷胱甘肽(GSH)和丙二醛(MDA)含量及观察肝组织病理学改变,以评价五味子提取物、黄芪多糖及其组合物对小鼠肝损伤的保护作用。结果:五味子提取物(270 mg/kg)和黄芪多糖(900 mg/kg)单独给药对对乙酰氨基酚肝损伤小鼠的血清ALT、AST和肝组织GSH和MDA没有显著性影响。30、90和270 mg/kg五味子提取物分别与100、300和900 mg/kg黄芪多糖配伍,可使对乙酰氨基酚肝损伤小鼠血清ALT、AST活性及肝组织MDA含量显著降低(P<0.05或P<0.01),肝组织GSH含量显著提高(P<0.05或P<0.01),肝组织细胞的病变程度得到明显改善。在高剂量配伍下,各指标的五味子提取物和黄芪多糖间相互作用指数CDI均小于0.7。结论:五味子提取物和黄芪多糖通过协同提高对乙酰氨基酚致肝损伤小鼠肝脏的还原性谷胱甘肽和抗氧化水平,降低血清ALT和AST水平,减轻肝组织细胞的损伤。

English Abstract

Synergistic protective effect of Fructus Schisandrae chinensis with Astragalus polysaccharides on paracetamol-induced acute liver injury in mice

Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

Received Date: 2009-01-06

Keywords:

Abstract: Objective : To study the synergistic protective effect and mechanism of extracts from Fructus Schisandrae chinensis(EFS) with astragalus polysaccharides(APS) on paracetamol-induced liver injury in mice. Methods :Mice liver injury was induced by peritoneal injection paracetamol physiologic saline solution at a dose of 500 mg/kg.The activities of serum alanine aminotransferase(ALT) and asparate aminotransferase(AST),the contents of malondialdehyde(MDA) and glutathione(GSH) in the liver tissue,and the liver histopathological changes were observed to evaluate the effects of EFS,APS and their combination on liver injury. Results : The EFS(270 mg/kg) or APS(900 mg/kg) alone did not produce any effect on liver injury induced by paracetamol in mice.The combination of EFS at dose of 30、90 and 270 mg/kg respectively with APS at dose of 100、300 and 900 mg/kg significantly decreased the activities of ALT and AST in serum and MAD level in liver tissue(P<0.05 or P<0.01),increased the GSH content in liver tissue(P<0.05 or P<0.01),and improved liver histopathological changes.The coefficients drug interaction(CDI) of related parameters between EFS(270 mg/kg) and APS(900 mg/kg) were all less than 0.7. Conclusion :The combination of EFS and APS reduced the ALT and AST level in serum,improved the liver histopathological changes of liver injury mice through synergistically increasing GSH content and antioxidant ability of liver.

燕菲, 张巧艳, 张宏, 韩婷, 焦磊, 秦路平. 五味子与黄芪多糖协同保护对乙酰氨基酚致小鼠急性肝损伤[J]. 药学实践与服务, 2009, 27(5): 340-344.

引用本文:

燕菲, 张巧艳, 张宏, 韩婷, 焦磊, 秦路平. 五味子与黄芪多糖协同保护对乙酰氨基酚致小鼠急性肝损伤[J]. 药学实践与服务, 2009, 27(5): 340-344.

Citation:

YAN Fei, ZHANG Qiao-yan, ZHANG Hong, HAN Ting, JIAO Lei, QIN Lu-ping. Synergistic protective effect of Fructus Schisandrae chinensis with Astragalus polysaccharides on paracetamol-induced acute liver injury in mice[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(5): 340-344.

全文HTML

下载: 全尺寸图片幻灯片

返回文章

用微信扫码二维码

分享至好友和朋友圈

主管、主办: 中国人民解放军海军军医大学地址: 上海市杨浦区国和路325号邮政编码: 200433

电话: （021）81871324,81871397 E-mail: yxsjzzs@163.com

网站备案号沪ICP备05003363号-1

本系统由北京仁和汇智信息技术有限公司开发

留言板

五味子与黄芪多糖协同保护对乙酰氨基酚致小鼠急性肝损伤

Synergistic protective effect of Fructus Schisandrae chinensis with Astragalus polysaccharides on paracetamol-induced acute liver injury in mice

计量

出版历程

五味子与黄芪多糖协同保护对乙酰氨基酚致小鼠急性肝损伤

第二军医大学药学院生药学教研室,上海200433

English Abstract

Synergistic protective effect of Fructus Schisandrae chinensis with Astragalus polysaccharides on paracetamol-induced acute liver injury in mice

Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

全文HTML

目录