毛细管区带电泳对5种三唑类化合物的手性分离
Chiral separation of five triazole antifungals by capillary zone electrophoresis
-
摘要: 目的: 考察毛细管区带电泳法(CZE)中各种因素对β-环糊精衍生物手性分离三唑类化合物的影响。 方法: 采用CZE对5个三唑类化合物的手性分离进行研究。BECKMAN P/ACE System 5 000毛细管电泳仪,紫外检测器。未涂渍熔融石英毛细管50μm×47 cm(有效长度40 cm);正极压力进样50 mbar×3 s,检测波长200 nm。β-环糊精的3种衍生物做为手性添加剂。 结果: 羧甲醚-β-环糊精为手性选择剂是5个化合物都能达到较好的手性分离,而硫酸酯-β-环糊精和二氟代羧甲醚-β-环糊精对这5个化合物无手性分离能力。在羧甲醚-β-环糊精环境下,优化选择30 mmol/L的NaH2PO4缓冲液含羧甲醚-β-环糊精5 mmol/L,用H3PO4调节pH2.2,温度20℃,电压20 KV,在此条件下5个化合物都能达到良好的手性分离。 结论: β-环糊精的种类和缓冲液的pH是影响这5对三唑类对映异构体分离的关键因素。Abstract: Objective: To investigate the factors that influence the chiral separation of triazole antifungals by capillary zone electrophoresis using β-cyclodextrin derivatives as chiral selector. Methods: Analyse were carried out on a P/ACE 5000 capillary electrophoresis system(Beckman,USA) with a UV detector,using an uncoated fused-silica capillary 50 μm × 47 cm with an effective length of 40 cm.Samples were injected into the capillary by 50 mbar pressure for 3 s.The detective wavelength was set at 200 nm.Three β-cyclodextrin derivatives were used as chiral selectors. Results: Satisfactory separation of the five triazole antifungals were achieved when using carboxymethylated-β-cyclodextrin(CM-β-CD) while the sulfobutyl-ether-β-cyclodextrin(SBE-β-CD) and difluoro-carboxymethylated-β-cyclodextrin showed no chiral separation ability to the five triazole antifungals.When using CM-β-CD,the enantiomeric separation of the five compounds and the best results were obtained in 30 mmol/L NaH2PO4 buffer(adjust to pH 2.2 with H3PO4)containing 5 mmol/L CM-β-CD.The voltage was 20 KV and the temperature was 20 ℃.The five triazole compounds were well separated on this condition. Conclusion: The type of β-CD and the pH of buffer is the major factor influencing the separation of the five triazole antifungals.
-
Key words:
- capillary zone electrophoresis /
- β-cyclodextrin derivative /
- triazole /
- chiral separation
计量
- 文章访问数: 1738
- HTML全文浏览量: 135
- PDF下载量: 474
- 被引次数: 0