高效液相色谱-蒸发光散射检测黄芪药材中黄芪甲苷的含量

纪松岗; 李翔; 朱东亮; 张国庆; 王彬; 娄子洋

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

高效液相色谱-蒸发光散射检测黄芪药材中黄芪甲苷的含量

纪松岗, 李翔, 朱东亮, 张国庆, 王彬, 娄子洋

文章导航 > 药学实践与服务 > 2005 > (5): 295-297

纪松岗, 李翔, 朱东亮, 张国庆, 王彬, 娄子洋. 高效液相色谱-蒸发光散射检测黄芪药材中黄芪甲苷的含量[J]. 药学实践与服务, 2005, (5): 295-297.

引用本文:

纪松岗, 李翔, 朱东亮, 张国庆, 王彬, 娄子洋. 高效液相色谱-蒸发光散射检测黄芪药材中黄芪甲苷的含量[J]. 药学实践与服务, 2005, (5): 295-297.

JI Song-gang, LI Xiang, ZHU Dong-liang, ZHANG Guo-qing, WANC Bin, LOU Zi-Yang. Determination astragaloside Ⅳ in Radix Astragali by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2005, (5): 295-297.

Citation:

JI Song-gang, LI Xiang, ZHU Dong-liang, ZHANG Guo-qing, WANC Bin, LOU Zi-Yang. Determination astragaloside Ⅳ in Radix Astragali by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2005, (5): 295-297.

高效液相色谱-蒸发光散射检测黄芪药材中黄芪甲苷的含量

1.
中国人民解放军第401医院药局, 山东, 青岛, 266071
2.
第二军医大学药学院药物分析教研室, 上海, 200433
3.
第二军医大学东方肝胆医院药局, 上海, 200438

基金项目: 上海市科委科技攻关基金(02419125)中药质量控制的现代化研究

Determination astragaloside Ⅳ in Radix Astragali by HPLC-ELSD

1.
Department of pharmacy, No. 401 Hospital of PLA, Qingdao 266071, China
2.
Department of pharmaceutical analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
3.
Department of Pharmacy, Eastern Hepatobthary Surgery Hospital, Shanghai 200438, China

摘要: 目的采用高效液相色谱-蒸发光散射法(HPLC-ELSD)测定膜荚黄芪中黄芪甲苷的含量方法黄芪药材经甲醇-氨水(v/v 9:1)超声提取,确定实验条件并进行方法学考察,色谱条件:依利特Hypersil ODS 2 色谱柱(4.6mm×250mm,5μm);流动相:A相为乙腈,B相为水,梯度洗脱;流速:1.0mL/min;柱温;室温;进样量:20μL.ELSD检测器条件:漂移管温度:40℃,载气(空气)压力:3.5Bar,增益值:7 结果方法学考察结果表明,黄芪甲苷低、中、高3个进样量的日内精密度和日间精密度的RSD均小于2.0%,48h内稳定性试验的RSD分别为1.24%,重复性试验的RSD分别为1.26%(n=5),最低检测限分别为0.6930mg/mL,加样回收率结果分别为97.05%,RSD=0.17%(n=3) 膜荚黄芪中黄芪甲苷的含量均符合药典标准讨论:该方法缩减了黄芪药材的前处理步骤,测定结果准确,能够用于黄芪药材中黄芪甲苷含量测定的研究
- HPLC-ELSD /
- 黄芪药材 /
- 黄芪甲苷 /
- 含量测定
Abstract: Objective To determine astragaloside Ⅳ in Radix Astragali by HPLC-KLSD. Methods Radix Astragali was extracted with MeOH-NH₃·H₂O(v/v 9:1),the condition was confirmed and the validation of the method was also tested. The chromatogra phy condition was with Hypersil ODS 2 column (4.6mm×250mm,5μm); mobile phase was A; ACN,B:H₂O, gradient elutron,flow speed was 1.0 ml/min, temperature of column was room temperature, inject volume:20μL. The KLSD conditions were as follows:the temperature of drift tube was 40℃, the gas pressure was 3.5Bar, the value of gain was 7. Results The intra-day and mter-day precision (RSD)at low, middle and high injection amount were all less than 2.0%. The stability (RSD) was 1.24% in 48h. The recuerence (RSD, n=5) was 1.26%. The limit of detection was 0.6930mg/mL The recoveries were 97.05% (KSD=0.17%, n=3) for astragaloside Ⅳ. The contents of astragaloside Ⅳ in (en different batch of Astragalus membranaceus (Fisch.) Bge. were accord to the standard of Chinese Pharmacopoeia. Conclusion The method covild shorten the process and time of Radix Astragali pretreatment with character of simple, suitable and reliable. The studies could apply to determine the content of astragaloside Ⅳ in Radix Astragalt.
- HPLC-ELSD /
- Radix Astragali /
- astragalosrde Ⅳ /
- determination

[1]	武菲菲, 张绡绮, 连敬, 杨晶, 翟蒙恩, 乔锐, 徐臣年, 杨婷婷. 黄芪甲苷激活ROCK/JNK介导自噬减轻异丙肾上腺素诱导的小鼠心肌纤维化 . 药学实践与服务, 2023, 41(8): 478-484. doi: 10.12206/j.issn.2097-2024.202212056
[2]	戴建英, 尤巍, 田甜, 洪战英. HPLC-ELSD法同时测定知母药材中5种成分的含量 . 药学实践与服务, 2022, 40(1): 34-37. doi: 10.12206/j.issn.1006-0111.202109070
[3]	刘璐, 于双雨, 刘艳华. HPLC-ELSD法测定胃康颗粒中人参皂苷Rb₁和黄芪甲苷的含量 . 药学实践与服务, 2020, 38(4): 359-363. doi: 10.12206/j.issn.1006-0111.202001083
[4]	韩凌, 王新霞, 吕磊, 张静如, 阚月一, 鲁莹, 傅翔. HPLC法同时测定多效防护霜中盐酸苯海拉明、甲氧基肉桂酸辛酯和水杨酸辛酯的含量 . 药学实践与服务, 2019, 37(6): 508-511. doi: 10.3969/j.issn.1006-0111.2019.06.007
[5]	宣伟东, 成熙, 叶曙娜, 徐德东. 用HPLC法测定百蕊草中3个黄酮苷的含量 . 药学实践与服务, 2019, 37(5): 460-463. doi: 10.3969/j.issn.1006-0111.2019.05.014
[6]	底雪梅, 袁曜晖, 张超, 高越. 黄芪甲苷抑制U937巨噬细胞CCL18表达及其作用机制研究 . 药学实践与服务, 2019, 37(1): 32-36,41. doi: 10.3969/j.issn.1006-0111.2019.01.008
[7]	付振贺, 赵亮, 周瑾, 王新霞, 张国庆, 黄玉凤. HPLC法测定扶正平消胶囊中芍药苷的含量 . 药学实践与服务, 2018, 36(1): 68-70,79. doi: 10.3969/j.issn.1006-0111.2018.01.014
[8]	杨梅, 李峻媛, 吴家彬, 玉秋萍, 韦敏灵, 韦红言. HPLC法测定积雪草配方颗粒中羟基积雪草苷和积雪草苷含量 . 药学实践与服务, 2017, 35(4): 359-361. doi: 10.3969/j.issn.1006-0111.2017.04.018
[9]	沈立茹, 李瑞宏, 王太亮. HPLC-ELSD法同时测定小儿宝泰康颗粒中贝母素甲与贝母素乙的含量 . 药学实践与服务, 2016, 34(4): 351-353. doi: 10.3969/j.issn.1006-0111.2016.04.017
[10]	曹金华, 谷莉, 吕磊, 李悦悦, 周闺臣, 赵亮, 张国庆. HPLC-ELSD法测定积雪草药材及配方颗粒剂中积雪草苷的含量 . 药学实践与服务, 2013, 31(5): 384-385,392. doi: 10.3969/j.issn.1006-0111.2013.05.019
[11]	尹茶, 吴婷婷, 朱东亮, 刘职瑞, 柴逸峰. HPLC-ELSD同时测定知母药材中4种主要皂苷的含量 . 药学实践与服务, 2012, 30(6): 433-436. doi: 10.3969/j.issn.1006-0111.2012.06.010
[12]	王志君, 尤本明, 孙华君, 宋洪杰, 刘继勇. RP-HPLC法测定雷公藤微乳中雷公藤内酯甲的含量 . 药学实践与服务, 2011, 29(4): 269-271.
[13]	李颜, 朱金辉, 李丹, 郭澄. RP-HPLC法测定中药木贼中黄酮醇苷的含量 . 药学实践与服务, 2011, 29(1): 33-34,37.
[14]	徐丽丽, 赵亮, 夏晖, 张国庆. RP-HPLC法测定重楼中四种甾体皂苷的含量 . 药学实践与服务, 2009, 27(3): 201-204.
[15]	陈俊, 黄玉凤, 王彬. HPLC-ELSD在中药分析及指纹图谱中的应用 . 药学实践与服务, 2009, 27(4): 251-253,310.
[16]	何厚罗, 黄章倍, 盛佳钰, 岳小强, 李敏, 辛海量. 人参茎叶总皂苷中人参皂苷Re的含量分析 . 药学实践与服务, 2009, 27(4): 291-293.
[17]	岳昌林, 郭忠. 高效液相色谱-蒸发光散射法测定白消安片的含量 . 药学实践与服务, 2006, (1): 25-27.
[18]	周永刚, 黄晟, 谷莉, 王彬, 张国庆, 娄子洋. HPLC法测定知母药材中芒果苷和新芒果苷的含量 . 药学实践与服务, 2005, (2): 99-102.
[19]	王建核. 高效液相色谱法测定脑脉通口服液中的黄芪甲苷 . 药学实践与服务, 2005, (6): 364-365.
[20]	王秀冬, 张兆旺, 孙秀梅, 吕青涛, 管华诗, 徐家敏. 薄层扫描法测定华海乙肝泡腾颗粒剂中黄芪甲苷的含量 . 药学实践与服务, 2001, (4): 226-228.

点击查看大图

计量

文章访问数: 1929
HTML全文浏览量: 163
PDF下载量: 613
被引次数: 0

高效液相色谱-蒸发光散射检测黄芪药材中黄芪甲苷的含量

1. 中国人民解放军第401医院药局, 山东, 青岛, 266071

2. 第二军医大学药学院药物分析教研室, 上海, 200433

3. 第二军医大学东方肝胆医院药局, 上海, 200438

基金项目: 上海市科委科技攻关基金(02419125)中药质量控制的现代化研究

收稿日期: 2005-06-12

关键词:

HPLC-ELSD /

黄芪药材 /

黄芪甲苷 /

含量测定

摘要: 目的采用高效液相色谱-蒸发光散射法(HPLC-ELSD)测定膜荚黄芪中黄芪甲苷的含量方法黄芪药材经甲醇-氨水(v/v 9:1)超声提取,确定实验条件并进行方法学考察,色谱条件:依利特Hypersil ODS 2 色谱柱(4.6mm×250mm,5μm);流动相:A相为乙腈,B相为水,梯度洗脱;流速:1.0mL/min;柱温;室温;进样量:20μL.ELSD检测器条件:漂移管温度:40℃,载气(空气)压力:3.5Bar,增益值:7 结果方法学考察结果表明,黄芪甲苷低、中、高3个进样量的日内精密度和日间精密度的RSD均小于2.0%,48h内稳定性试验的RSD分别为1.24%,重复性试验的RSD分别为1.26%(n=5),最低检测限分别为0.6930mg/mL,加样回收率结果分别为97.05%,RSD=0.17%(n=3) 膜荚黄芪中黄芪甲苷的含量均符合药典标准讨论:该方法缩减了黄芪药材的前处理步骤,测定结果准确,能够用于黄芪药材中黄芪甲苷含量测定的研究

English Abstract

Determination astragaloside Ⅳ in Radix Astragali by HPLC-ELSD

1. Department of pharmacy, No. 401 Hospital of PLA, Qingdao 266071, China

2. Department of pharmaceutical analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

3. Department of Pharmacy, Eastern Hepatobthary Surgery Hospital, Shanghai 200438, China

Received Date: 2005-06-12

Keywords:

Abstract: Objective To determine astragaloside Ⅳ in Radix Astragali by HPLC-KLSD. Methods Radix Astragali was extracted with MeOH-NH₃·H₂O(v/v 9:1),the condition was confirmed and the validation of the method was also tested. The chromatogra phy condition was with Hypersil ODS 2 column (4.6mm×250mm,5μm); mobile phase was A; ACN,B:H₂O, gradient elutron,flow speed was 1.0 ml/min, temperature of column was room temperature, inject volume:20μL. The KLSD conditions were as follows:the temperature of drift tube was 40℃, the gas pressure was 3.5Bar, the value of gain was 7. Results The intra-day and mter-day precision (RSD)at low, middle and high injection amount were all less than 2.0%. The stability (RSD) was 1.24% in 48h. The recuerence (RSD, n=5) was 1.26%. The limit of detection was 0.6930mg/mL The recoveries were 97.05% (KSD=0.17%, n=3) for astragaloside Ⅳ. The contents of astragaloside Ⅳ in (en different batch of Astragalus membranaceus (Fisch.) Bge. were accord to the standard of Chinese Pharmacopoeia. Conclusion The method covild shorten the process and time of Radix Astragali pretreatment with character of simple, suitable and reliable. The studies could apply to determine the content of astragaloside Ⅳ in Radix Astragalt.

纪松岗, 李翔, 朱东亮, 张国庆, 王彬, 娄子洋. 高效液相色谱-蒸发光散射检测黄芪药材中黄芪甲苷的含量[J]. 药学实践与服务, 2005, (5): 295-297.

引用本文:

纪松岗, 李翔, 朱东亮, 张国庆, 王彬, 娄子洋. 高效液相色谱-蒸发光散射检测黄芪药材中黄芪甲苷的含量[J]. 药学实践与服务, 2005, (5): 295-297.

Citation:

全文HTML

下载: 全尺寸图片幻灯片

返回文章

用微信扫码二维码

分享至好友和朋友圈

主管、主办: 中国人民解放军海军军医大学地址: 上海市杨浦区国和路325号邮政编码: 200433

电话: （021）81871324,81871397 E-mail: yxsjzzs@163.com

网站备案号沪ICP备05003363号-1

本系统由北京仁和汇智信息技术有限公司开发

留言板

高效液相色谱-蒸发光散射检测黄芪药材中黄芪甲苷的含量

Determination astragaloside Ⅳ in Radix Astragali by HPLC-ELSD

计量

出版历程

高效液相色谱-蒸发光散射检测黄芪药材中黄芪甲苷的含量

1. 中国人民解放军第401医院药局, 山东, 青岛, 266071 2. 第二军医大学药学院药物分析教研室, 上海, 200433 3. 第二军医大学东方肝胆医院药局, 上海, 200438

English Abstract

Determination astragaloside Ⅳ in Radix Astragali by HPLC-ELSD

1. Department of pharmacy, No. 401 Hospital of PLA, Qingdao 266071, China 2. Department of pharmaceutical analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, China 3. Department of Pharmacy, Eastern Hepatobthary Surgery Hospital, Shanghai 200438, China

全文HTML

目录

1. 中国人民解放军第401医院药局, 山东, 青岛, 266071

2. 第二军医大学药学院药物分析教研室, 上海, 200433

3. 第二军医大学东方肝胆医院药局, 上海, 200438

1. Department of pharmacy, No. 401 Hospital of PLA, Qingdao 266071, China

2. Department of pharmaceutical analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

3. Department of Pharmacy, Eastern Hepatobthary Surgery Hospital, Shanghai 200438, China