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四逆汤治疗甲状腺功能减退症的血清代谢组学研究

贾雨婷 靳雨晨 谭光国

贾雨婷, 靳雨晨, 谭光国. 四逆汤治疗甲状腺功能减退症的血清代谢组学研究[J]. 药学实践与服务, 2016, 34(3): 237-240,248. doi: 10.3969/j.issn.1006-0111.2016.03.011
引用本文: 贾雨婷, 靳雨晨, 谭光国. 四逆汤治疗甲状腺功能减退症的血清代谢组学研究[J]. 药学实践与服务, 2016, 34(3): 237-240,248. doi: 10.3969/j.issn.1006-0111.2016.03.011
JIA Yuting, JIN Yuchen, TAN Guangguo. Study on Sini decoction in treatment of hypothyroidism by metabonomic method[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 237-240,248. doi: 10.3969/j.issn.1006-0111.2016.03.011
Citation: JIA Yuting, JIN Yuchen, TAN Guangguo. Study on Sini decoction in treatment of hypothyroidism by metabonomic method[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 237-240,248. doi: 10.3969/j.issn.1006-0111.2016.03.011

四逆汤治疗甲状腺功能减退症的血清代谢组学研究

doi: 10.3969/j.issn.1006-0111.2016.03.011
基金项目: 国家自然科学基金项目(81402888);全军医学科技青年培育项目(13QNT197);陕西自然科学基金项目(2015JM8458)

Study on Sini decoction in treatment of hypothyroidism by metabonomic method

  • 摘要: 目的 利用血清代谢组学鉴定甲状腺功能减退症(甲减)相关的代谢变化,评价四逆汤治疗甲减的作用。 方法 将24只大鼠分为假手术组、模型组和四逆汤治疗组,采用甲状腺切除诱导甲减模型,给予4周相应的治疗后,取大鼠血清进行超高效液相色谱-四极杆-飞行时间质谱仪(UPLC-Q-TOFMS)分析,结合模式识别分析各组间代谢物差异,评价四逆汤的治疗作用。 结果 血清代谢组学分析鉴定了9种甲减相关的生物标志物,涉及三羧酸循环、苯丙氨酸代谢、鞘脂代谢和磷脂代谢,同时数据显示四逆汤对甲减有改善治疗作用。 结论 四逆汤能够通过调节失衡的三羧酸循环、苯丙氨酸代谢和磷脂代谢而发挥治疗甲减的作用。
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    [2] Flynn RW, Bonellie SR, Jung RT, et al. Serum thyroid-stimulating hormone concentration and morbidity from cardiovascular disease and fractures in patients on long-term thyroxine therapy[J]. J Clin Endocrinol Metab, 2010,95(1):186-193.
    [3] La Vignera S, Vicari E, Tumino S, et al. L-thyroxin treatment and post-menopausal osteoporosis:relevance of the risk profile present in clinical history[J]. Minerva Ginecol, 2008,60(6):475-484.
    [4] 蔡定芳, 陈晓红, 刘彦芳, 等. 探讨阴中求阳与阳中求阴对谷氨酸单钠大鼠下丘脑-垂体-肾上腺-胸腺轴的影响[J]. 中国中西医结合杂志,1999,19(7):415-417.
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    [8] Wu S, Tan G, Dong X, et al. Metabolic profiling provides a system understanding of hypothyroidism in rats and its application[J]. PLoS One, 2013, 8(2):e55599.
    [9] 国家药典委员会. 中华人民共和国药典2015年版一部[S].北京:中国医药科技出版社,2015:789.
    [10] Alzoubi KH, Gerges NZ, Aleisa AM, et al. Levothyroxin restores hypothyroidism-induced impairment of hippocampus-dependent learning and memory:Behavioral, electrophysiological, and molecular studies[J]. Hippocampus, 2009, 19(1):66-78.
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    [13] Sjoberg S, Eriksson M, Nordin C. L-thyroxine treatment and neurotransmitter levels in the cerebrospinal fluid of hypothyroid patients:a pilot study[J]. Eur J Endocrinol, 1998, 139(5):493-497.
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  • [1] 景凯, 杨慈荣, 张圳, 臧艺蓓, 刘霞.  黄芪甲苷衍生物治疗慢性心力衰竭小鼠的药效评价及作用机制研究 . 药学实践与服务, 2024, 42(5): 190-197. doi: 10.12206/j.issn.2097-2024.202310004
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  • 收稿日期:  2016-03-14
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四逆汤治疗甲状腺功能减退症的血清代谢组学研究

doi: 10.3969/j.issn.1006-0111.2016.03.011
    基金项目:  国家自然科学基金项目(81402888);全军医学科技青年培育项目(13QNT197);陕西自然科学基金项目(2015JM8458)

摘要: 目的 利用血清代谢组学鉴定甲状腺功能减退症(甲减)相关的代谢变化,评价四逆汤治疗甲减的作用。 方法 将24只大鼠分为假手术组、模型组和四逆汤治疗组,采用甲状腺切除诱导甲减模型,给予4周相应的治疗后,取大鼠血清进行超高效液相色谱-四极杆-飞行时间质谱仪(UPLC-Q-TOFMS)分析,结合模式识别分析各组间代谢物差异,评价四逆汤的治疗作用。 结果 血清代谢组学分析鉴定了9种甲减相关的生物标志物,涉及三羧酸循环、苯丙氨酸代谢、鞘脂代谢和磷脂代谢,同时数据显示四逆汤对甲减有改善治疗作用。 结论 四逆汤能够通过调节失衡的三羧酸循环、苯丙氨酸代谢和磷脂代谢而发挥治疗甲减的作用。

English Abstract

贾雨婷, 靳雨晨, 谭光国. 四逆汤治疗甲状腺功能减退症的血清代谢组学研究[J]. 药学实践与服务, 2016, 34(3): 237-240,248. doi: 10.3969/j.issn.1006-0111.2016.03.011
引用本文: 贾雨婷, 靳雨晨, 谭光国. 四逆汤治疗甲状腺功能减退症的血清代谢组学研究[J]. 药学实践与服务, 2016, 34(3): 237-240,248. doi: 10.3969/j.issn.1006-0111.2016.03.011
JIA Yuting, JIN Yuchen, TAN Guangguo. Study on Sini decoction in treatment of hypothyroidism by metabonomic method[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 237-240,248. doi: 10.3969/j.issn.1006-0111.2016.03.011
Citation: JIA Yuting, JIN Yuchen, TAN Guangguo. Study on Sini decoction in treatment of hypothyroidism by metabonomic method[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 237-240,248. doi: 10.3969/j.issn.1006-0111.2016.03.011
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